Archive | October, 2013

Arachidonyl ethanolamide induces apoptosis of uterine cervix cancer cells via aberrantly expressed vanilloid receptor-1.

Arachidonyl ethanolamide induces apoptosis of uterine cervix cancer cells via aberrantly expressed vanilloid receptor-1. Contassot E, Tenan M, Schnüriger V, Pelte MF, Dietrich PY. Source Oncology Division, Laboratory of Tumor Immunology, University Hospital, Geneva, Switzerland. Abstract OBJECTIVES: Delta(9)-Tetrahydrocannabinol, the active agent of Cannabis sativa, exhibits well-documented antitumor properties, but little is known about the possible effects mediated by endogenous cannabinoids on human tumors. […]

Effects of delta-9-tetrahydrocannabinol on cultured HeLa cell growth and development.

Effects of delta-9-tetrahydrocannabinol on cultured HeLa cell growth and development. Blevins RD, Smith DP. Abstract Monolayer cultures of HeLa cells were used to monitor the effects of non-lethal concentrations of delta-9-tetrahydrocannabinol (delta-9-THC) on the pool sizes of the acid-soluble and acid-insoluble DNA moieties and cytoplasmic RNA pool sizes. The DNA fractions were separated using acid precipitation and low speed centrifugation, […]

Delta9-tetrahydrocannabinol increases C6 glioma cell death produced by oxidative stress.

Delta9-tetrahydrocannabinol increases C6 glioma cell death produced by oxidative stress. Goncharov I, Weiner L, Vogel Z. Source Department of Neurobiology, Weizmann Institute of Science, Herzel Street, Rehovot 76100, Israel. igor.goncharov@weizmann.ac.il Abstract (-)Delta9-tetrahydrocannabinol is a scavenger of free radicals. However, the activation of the CB1 receptor in cultured C6 glioma cells by (-)delta9-tetrahydrocannabinol in the presence of reagents generating reactive […]

Inhibition of cancer cell invasion by cannabinoids via increased expression of tissue inhibitor of matrix metalloproteinases-1.

Inhibition of cancer cell invasion by cannabinoids via increased expression of tissue inhibitor of matrix metalloproteinases-1. Ramer R, Hinz B. Source Institute of Toxicology and Pharmacology, University of Rostock, Schillingallee 70, Rostock D-18057, Germany. Abstract BACKGROUND: Cannabinoids, in addition to having palliative benefits in cancer therapy, have been associated with anticarcinogenic effects. Although the antiproliferative activities of cannabinoids have been […]

Activation of transient receptor potential A1 channels by mustard oil, tetrahydrocannabinol and Ca2+ reveals different functional channel states.

Activation of transient receptor potential A1 channels by mustard oil, tetrahydrocannabinol and Ca2+ reveals different functional channel states. Cavanaugh EJ, Simkin D, Kim D. Source Department of Physiology and Biophysics, Rosalind Franklin University of Medicine and Science, Chicago Medical School, 3333 Green Bay Road, North Chicago, IL 60064, USA. Abstract Our recent study has shown that activation […]

Acylamido analogs of endocannabinoids selectively inhibit cancer cell proliferation.

Acylamido analogs of endocannabinoids selectively inhibit cancer cell proliferation. Burstein S, Salmonsen R. Source Department of Biochemistry and Molecular Pharmacology, The University of Massachusetts Medical School, Worcester, MA 01605, USA. sumner.burstein@umassmed.edu Abstract A series of amide derivatives of long-chain fatty acids has been studied for their effects on the proliferation of cancer cells in vitro. Fatty acids ranged […]

Fatty Acid-binding Proteins Transport N-Acylethanolamines to Nuclear Receptors and Are Targets of Endocannabinoid Transport Inhibitors*

J Biol Chem. 2012 January 27; 287(5): 3415–3424. Published online 2011 December 14. doi:  10.1074/jbc.M111.304907 PMCID: PMC3270995 Fatty Acid-binding Proteins Transport N-Acylethanolamines to Nuclear Receptors and Are Targets of Endocannabinoid Transport Inhibitors* Martin Kaczocha,‡,1 Stephanie Vivieca,‡ Jing Sun,‡ Sherrye T. Glaser,§ and Dale G. Deutsch‡ Author information ► Article notes ► Copyright and License information ► This article has been cited by other articles in PMC. Go to: Abstract N-Acylethanolamines (NAEs) are bioactive […]

Endocannabinoids Stimulate Human Melanogenesis via Type-1 Cannabinoid Receptor*

  J Biol Chem. 2012 May 4; 287(19): 15466–15478. Published online 2012 March 19. doi:  10.1074/jbc.M111.314880 PMCID: PMC3346111 Endocannabinoids Stimulate Human Melanogenesis via Type-1 Cannabinoid Receptor* Mariangela Pucci,‡,1 Nicoletta Pasquariello,‡,1 Natalia Battista,‡§,1 Monia Di Tommaso,‡Cinzia Rapino,‡ Filomena Fezza,§¶ Michela Zuccolo,‖ Roland Jourdain,‖ Alessandro Finazzi Agrò,¶** Lionel Breton,‖ and Mauro Maccarrone‡§,2 Author information ► Article notes ► Copyright and License information ► This article has been cited by other articles in PMC. Go to: Abstract We show that […]

Differential β-arrestin2 requirements for constitutive and agonist-induced internalization of the CB1 cannabinoid receptor.

Differential β-arrestin2 requirements for constitutive and agonist-induced internalization of the CB1 cannabinoid receptor. Gyombolai P, Boros E, Hunyady L, Turu G. Source Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary. pal.gyombolai@eok.sote.hu Abstract CB1 cannabinoid receptor (CB1R) undergoes both constitutive and agonist-induced internalization, but the underlying mechanisms of these processes and the role of β-arrestins in the regulation of CB1R function […]

R(+)-methanandamide-induced apoptosis of human cervical carcinoma cells involves a cyclooxygenase-2-dependent pathway.

R(+)-methanandamide-induced apoptosis of human cervical carcinoma cells involves a cyclooxygenase-2-dependent pathway. Eichele K, Ramer R, Hinz B. Source Institute for Toxicology and Pharmacology, University of Rostock, Schillingallee 70, D-18057, Rostock, Germany. Abstract PURPOSE: Cannabinoids have received renewed interest due to their antitumorigenic effects. Using human cervical carcinoma cells (HeLa), this study investigates the role of cyclooxygenase-2 (COX-2) in apoptosis elicited by the […]