Canna~Fangled Abstracts

Analysis of Cannabinoids Concentration in Cannabis Oil Galenic Preparations: Harmonization between Three Laboratories in Northern Italy

By May 14, 2021June 6th, 2021No Comments

doi: 10.3390/ph14050462.

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Free PMC article

Abstract

Medical cannabis is increasingly being used in the treatment and support of several diseases and syndromes. The quantitative determination of active ingredients (delta-9 tetrahydrocannabinol, THC, and cannabidiol, CBD) in galenic oily preparations is prescribed by law for each produced batch. The aim of this work is to describe the organization of the titration activity centralized at three regional reference laboratories in Northern Italy. Pre-analytical, analytical, and post-analytical phases have been defined in order to guarantee high quality standards. A cross-validation between laboratories allowed for the definition of the procedures that guarantee the interchangeability between reference laboratories. The risk management protocol adopted can be useful for others who need to undertake this activity.

 

Keywords: UHPLC/MS-MS, cannabinoids stability, cannabis oil, medical cannabis

Conflict of interest statement

A.D. and A.M. declare to be shareholder of CoQua Lab. The other authors declare no conflict of interest.

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References

    1. Ufficiale G. Decreto Ministeriale 9 Novembre 2015. Funzioni di Organisimo Statale per la Cannabis Previsto dagli Articoli 23 e 28 della Convenzione Unica Sugli Stupefacenti del 1961, Come Modificata nel 1972. [(accessed on 26 April 2021)]; Available online: https://www.epicentro.iss.it/farmaci/pdf/Decreto%20uso%20medico%20Cannab….
    1. Degenhardt F., Stehle F., Kayser O. The biosynthesis of Cannabinoids. In: Preedy V., editor. Handbook of Cannabis and Related Pathologies: Biology, Pharmacology, Diagnosis and Treatment. Elsevier; Amsterdam, The Netherlands: 2017.
    1. Elsohly M.A., Slade D. Chemical constituents of marijuana: The complex mixture of natural cannabinoids. Life Sci. 2005;78:539–548. doi: 10.1016/j.lfs.2005.09.011. – DOI – PubMed
    1. Gurney S.M., Scott K.S., Kacinko S.L., Presley B.C., Logan B.K. Pharmacology, Toxicology, and Adverse Effects of Synthetic Cannabinoid Drugs. Forensic Sci. Rev. 2014;26:53–78. – PubMed
    1. Russo E.B., McPartland J.M. Cannabis is more than simply delta(9)-tetrahydrocannabinol. Psychopharmacol. Berl. 2003;165:431–432. doi: 10.1007/s00213-002-1348-z. – DOI – PubMed

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