Canna~Fangled Abstracts

An Optimized Terpene Profile for a New Medical Cannabis Oil

By January 27, 2022February 28th, 2022No Comments

. 2022 Jan 27;14(2):298.

doi: 10.3390/pharmaceutics14020298.

Valentina Maggini 1, Lorenzo Calvi 1, Tommaso Pelagatti 1 2, Eugenia Rosaria Gallo 1, Celine Civati 2, Carlo Privitera 3, Flavio Squillante 2, Paolo Maniglia 4, Domenico Di Candia 5, Roberto Spampatti 2, Fabio Firenzuoli 1
Affiliations 

Free PMC article

Abstract

The purpose of this analytical study was to develop an advanced formulation of medical Cannabis oil (MCO) comparing the chemical profile of different extracts obtained with two existing methods (SIFAP and CALVI) and one original upgraded (CERFIT) method. Preparation methods were applied with varying solvent, temperature, and duration of the decarboxylation and extraction steps. HPLC-MS/MS TSQ and GC/FID-HS analyses were performed to investigate cannabinoid and terpene contents in the three oil extracts. Cannabinoids profile remained comparable between the formulations. CERFIT extracts exhibited a superior quantity of total terpene hydrocarbon forms (e.g., limonene and α-pinene) with no degradation occurrence (i.e., oxidized terpenes not quantifiable). Thus, this new method optimized the phytochemical profile of the MCO presenting a value opportunity to obtain a standardized high-level therapeutic product.

Keywords: Cannabis sativa L., cannabinoids, extraction method, medical use, monoterpenes, oil preparation, oxidized terpenes, sesquiterpenes

Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

References

    1. Crocq M.-A. History of Cannabis and the Endocannabinoid System. Dialogues Clin. Neurosci. 2020;22:223–228. doi: 10.31887/DCNS.2020.22.3/mcrocq. – DOI – PMC – PubMed
    1. Heng M., McTague M.F., Lucas R.C., Harris M.B., Vrahas M.S., Weaver M.J. Patient Perceptions of the Use of Medical Marijuana in the Treatment of Pain After Musculoskeletal Trauma: A Survey of Patients at 2 Trauma Centers in Massachusetts. J. Orthop. Trauma. 2018;32:e25–e30. doi: 10.1097/BOT.0000000000001002. – DOI – PubMed
    1. Kosiba J.D., Maisto S.A., Ditre J.W. Patient-Reported Use of Medical Cannabis for Pain, Anxiety, and Depression Symptoms: Systematic Review and Meta-Analysis. Soc. Sci. Med. 2019;233:181–192. doi: 10.1016/j.socscimed.2019.06.005. – DOI – PubMed
    1. Aran A., Harel M., Cassuto H., Polyansky L., Schnapp A., Wattad N., Shmueli D., Golan D., Castellanos F.X. Cannabinoid Treatment for Autism: A Proof-of-Concept Randomized Trial. Mol. Autism. 2021;12:6. doi: 10.1186/s13229-021-00420-2. – DOI – PMC – PubMed
    1. Uddin M.S., Mamun A.A., Sumsuzzman D.M., Ashraf G.M., Perveen A., Bungau S.G., Mousa S.A., El-Seedi H.R., Bin-Jumah M.N., Abdel-Daim M.M. Emerging Promise of Cannabinoids for the Management of Pain and Associated Neuropathological Alterations in Alzheimer’s Disease. Front. Pharmacol. 2020;11:1097. doi: 10.3389/fphar.2020.01097. – DOI – PMC – PubMed

LinkOut – more resources

Leave a Reply