2016;1412:85-93. doi: 10.1007/978-1-4939-3539-0_9.
Abstract
The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.
KEYWORDS:
3-Isobutyl-1-methylxanthine (IBMX); Agonist; Antagonist; Cannabinoid receptor type 2; Cyclic AMP; Forskolin (FSK); Inverse agonist
- PMID: 27245894
- [PubMed – in process]
