- PMID: 32456251
- DOI: 10.3390/plants9050665
Abstract
Pollen viability and storage is of great interest to cannabis breeders and researchers to maintain desirable germplasm for future use in breeding or for biotechnological and gene editing applications. Here, we report a simple and efficient cryopreservation method for long-term storage of Cannabis sativa pollen. Additionally, the bicellular nature of cannabis pollen was identified using DAPI (4′,6-diamidino-2-phenylindole) staining. A pollen germination assay was developed to assess cannabis pollen viability and used to demonstrate that pollen collected from different principal growth stages exhibited differential longevity. Finally, a simple and efficient method that employs pollen combined with baked whole wheat flour and subsequent desiccation under vacuum was developed for the long-term cryopreservation of C. sativa pollen. Using this method, pollen viability was maintained in liquid nitrogen after four months, suggesting long-term preservation of cannabis pollen.
Keywords: Cannabis sativa, DAPI staining of germinated pollen, cryopreservation, long-term storage, pollen germination assay, sperm nuclei, vegetative nuclei
Similar articles
-
Storage and Viability Assessment of Date Palm Pollen.
Methods Mol Biol. 2017;1638:3-13. doi: 10.1007/978-1-4939-7159-6_1.PMID: 28755209 -
Ann Bot. 2014 Sep;114(3):561-9. doi: 10.1093/aob/mcu139. Epub 2014 Jul 8.PMID: 25006180 Free PMC article.
-
BMC Genomics. 2010 May 28;11:338. doi: 10.1186/1471-2164-11-338.PMID: 20507633 Free PMC article.
-
Effect of low storage temperature on pollen viability of fifteen herbaceous peonies.
Biotechnol Rep (Amst). 2019 Jan 17;21:e00309. doi: 10.1016/j.btre.2019.e00309. eCollection 2019 Mar.PMID: 30775270 Free PMC article. Review. -
In vitro conservation of Dendrobium germplasm.
Plant Cell Rep. 2014 Sep;33(9):1413-23. doi: 10.1007/s00299-014-1631-6. Epub 2014 May 21.PMID: 24845051 Review.