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Canna~Fangled Abstracts

Therapeutic Role of Hemp (Cannabis sativa) Against Copper-Induced Toxicity in Labeo rohita and Cirrhinus mrigala

By April 3, 2023No Comments

. 2023 Apr 3.

doi: 10.1007/s12011-023-03650-y.

Online ahead of print.
Azam Jan Afridi 1, Amina Zuberi 2, Ali Muhammad Yousafzai 3, Muhammad Kamran 4
Affiliations 

Abstract

For decades hemp has been used as a therapeutic agent for enhancing immunity in animals. Current study was conceptualized to find out the protective role of dietary hemp seed products (hemp seed oil (HO) and hemp seed (HS)) against copper-induced toxicity in fish. Fingerlings of Labeo rohita (Rohu) and Cirrhinus mrigala (Mrigal) were exposed to copper at 20% of the 96 h LC50 (1.34 and 1.52 ppm, respectively) for 30 days. Following Cu exposure, fish were maintained on two types of hemp (Cannabis sativa)-supplemented feeds, on graded levels of hemp seed oil (HO: 1%, 2%, 3%) and hemp seed (HS: 5%, 10%, 15%) for 50 days, while one group was the control (without any copper exposure as well as any supplementation). Copper exposure significantly increased (P < 0.05) WBCs, hematocrit, MCHV, eosinophils, and lymphocytes in L. rohita and also in C. mrigala as compared to control. Copper exposure also significantly (P < 0.05) changed lysozymes, plasma protein, and IgM in both species, in comparison to control. Moreover, alkaline phosphatase, bilirubin, serum glutamic-pyruvic transaminase, and aspartate transaminase were significantly (P < 0.05) changed by copper exposure in comparison to control in both species. Additionally, Antioxidant enzymes like catalase, superoxide dismutase, glutathione reductase, and glutathione peroxidase were also significantly (P < 0.05) increased in the brain, gills, liver, and muscle of copper-exposed group in both species as compared to control. Interestingly, all the altered parameter of blood, serum, liver function tests, and antioxidant enzymes (in different organs) because of copper toxicity were successfully reverted to normal level in hemp seed oil (HO) and hemp seed (HS)-supplemented fed groups of both species. In conclusion, hemp seed supplementation showed significant (P < 0.05) improved results against copper toxicity. Thus, it could be recommended as an animal feed ingredient for its therapeutic role.

Keywords: Cirrhinus mrigala, Copper toxicity, Hemp seed products, Labeo rohita, Therapeutic role

References

    1. Murtaza MUH, Zuberi A, Ahmad M, Amir I, Kamran M, Ahmad M (2020) Influence of early rearing environment on water-borne cortisol and expression of stress-related genes in grass carp (Ctenopharyngodon idella). Mol Biol Rep 47:5051–5060 – PubMed
    1. Afridi AJ, Zuberi A, Yousafzai AM, Kamran M, Ullah S (2019) Hemp (Marijuana) reverted copper-induced toxic effects on the essential fatty acid profile of Labeo rohita and Cirrhinus mrigala. Mol Biol Rep 46:391–401 – PubMed
    1. Kamran M, Razzaq H, Noorullah M, Ahmad M, Zuberi A (2023) Comparative analysis of genetic diversity, growth performance, disease resistance and expression of growth and immune related genes among five different stocks of Labeo rohita (Hamilton, 1822). Aquaculture 739277:1–13
    1. Kamran M, Yaqub A, Malkani N, Anjum KM, Awan MN, Paknejad H (2020) Identification and phylogenetic analysis of Channa species from riverine system of Pakistan using COI gene as a DNA barcoding marker. J Bioresour Manag 7(2):10
    1. Yaqub A, Awan MN, Kamran M, Majeed I (2021) Evaluation of potential applications of dietary probiotic (Bacillus licheniformis SB3086): effect on growth, digestive enzyme activity, hematological, biochemical, and immune response of Tilapia (Oreochromis mossambicus). Turk J Fish Aquat Sci 22(5):1–12

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