Implementation of a generic liquid chromatographic method development workflow: Application to the analysis of phytocannabinoids and Cannabis sativa extracts.

J Pharm Biomed Anal. 2018 Mar 31;155:116-124. doi: 10.1016/j.jpba.2018.03.059.

[Epub ahead of print]

Fekete S¹, Sadat-Noorbakhsh V², Schelling C², Molnár I³, Guillarme D², Rudaz S², Veuthey JL².

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Highlights

• A generic RPLC method development workflow was proposed.
• Screening, optimization, virtual refinement and virtual robustness testing were done.
• This workflow was applied to phytocannabinoids and Cannabis sativa extracts.
• This whole procedure takes only around 4 days of work.

Abstract

A generic liquid chromatographic method development workflow was developed and successfully applied to the analysis of phytocannabinoids and Cannabis sativa extracts. Our method development procedure consists in four steps: At the end, this method development workflow was performed in less than 4 days and minimizes the costs of the method development in liquid chromatography.