Direct modulation of the outer mitochondrial membrane channel, voltage-dependent anion channel 1 (VDAC1) by cannabidiol: a novel mechanism for cannabinoid-induced cell death
Abstract
Cannabidiol (CBD) is a non-psychoactive plant cannabinoid that inhibits cell proliferation and induces cell death of cancer cells and activated immune cells. It is not an agonist of the classical CB1/CB2 cannabinoid receptors and the mechanism by which it functions is unknown. Here, we studied the effects of CBD on various mitochondrial functions in BV-2 microglial cells. Our findings indicate that CBD treatment leads to a biphasic increase in intracellular calcium levels and to changes in mitochondrial function and morphology leading to cell death. Density gradient fractionation analysis by mass spectrometry and western blotting showed colocalization of CBD with protein markers of mitochondria. Single-channel recordings of the outer-mitochondrial membrane protein, the voltage-dependent anion channel 1 (VDAC1) functioning in cell energy, metabolic homeostasis and apoptosis revealed that CBD markedly decreases channel conductance. Finally, using microscale thermophoresis, we showed a direct interaction between purified fluorescently labeled VDAC1 and CBD. Thus, VDAC1 seems to serve as a novel mitochondrial target for CBD. The inhibition of VDAC1 by CBD may be responsible for the immunosuppressive and anticancer effects of CBD.
Introduction
The non-psychoactive plant cannabinoid, cannabidiol (CBD), in a 1:1 mixture with the psychoactive plant cannabinoid, Δ9-tetrahydrocannabinol (THC), has been clinically approved for the treatment of neuropathic pain and spasticity in multiple sclerosis.1 CBD alone has strong anti-inflammatory and immunosuppressive effects in diverse animal models of disease such as diabetes,2 cancer,3 rheumatoid arthritis4 and multiple sclerosis.5 In addition, CBD has been reported to have anxiolytic, antiemetic and antipsychotic effects.6, 7, 8 Moreover, CBD has been shown to possess antitumor activity in human breast carcinoma and to effectively reduce primary tumor mass, as well as size and number of lung metastasis in preclinical animal models of breast cancer.3, 9 CBD is not an agonist of the classical cannabinoid receptor CB1 and therefore does not induce any of the unwanted psychoactive effects observed with THC. While the molecular targets of CBD are elusive, these reports incite growing clinical interest in the use of CBD to treat these diseases.10
Numerous molecular targets of CBD have been described depending on the experimental models used, among them are the GPCRs: GPR55, GPR18, and 5HT1A receptors (for review, refer to previous studies8, 11). In addition, CBD has been shown to bind to the equilibrative nucleotide transporter affecting the activity of A2A adenosine receptors.12 CBD was also shown to activate various transient receptor potential channels (TRPV1, TRPV2, TRPM8, TRPA1; ref. 13), as well as the nuclear peroxisome proliferator-activating receptor γ (PPARγ).14 Furthermore, Ryan et al.15 reported the involvement of a specific mitochondrial target for CBD in hippocampal neurons and glia, the mitochondrial sodium-calcium exchanger. In these cells CBD was neuroprotective and did not affect cell viability.15, 16
While CBD was reported as a neuroprotective agent in many in vitro and in vivo models,15, 17, 18 it has been shown to induce cell death in cancer cells and several types of immune cells. In cancer models, CBD induced cell death in breast cancer cells (MDA-MB-231; MCF-7, SK-BR3, ZR-75-1) via mitochondria-mediated signaling pathways as characterized by swollen mitochondria, reduced mitochondrial membrane potential, and increased reactive oxygen species (ROS) production.19 Additionally, CBD was shown to induce cell death in thymoma cells, leukemic cell lines, and gliomas.17, 20, 21, 22, 23
In immune cells, CBD induced cell death in primary murine thymocytes and splenocytes.23, 24 In human monocytes CBD has shown mixed effects on cell death depending on time in culture and the anti-oxidative capacity of the cells.25 Moreover, CBD has been shown to induce cell death in mouse peritoneal macrophages,26 BV-2 microglial cells,27 and murine microglial cells.28
Using the BV-2 microglial cell model, we reported that CBD-induced cell death can be prevented by the cholesterol chelating/lipid raft disrupting agent methyl-β-cyclodextrin.27 Similar results were reported by Wu et al.28 for murine microglia, supporting the idea that CBD targets analogous molecular mechanisms in BV-2 and primary microglial cells. We have also demonstrated that CBD stimulates the transcription of genes regulating cellular stress responses, induces anti-inflammatory effects (by downregulating the expression of proinflammatory genes and upregulating anti-inflammatory mediators) and modulates the transcripts of cholesterol-related enzymes.27, 29, 30, 31, 32, 33
Here we report the identification of a new mitochondrial molecular target for CBD, the voltage-dependent anion channel 1 (VDAC1). VDAC1 is a multifunctional channel located in the mitochondrial outer membrane (OMM). It has a central role in controlling cellular energy and metabolism by mediating the transfer of metabolites between the mitochondria and the cytosol.34 Moreover, VDAC1 is a key factor in mitochondria-mediated apoptosis, participating in the release of mitochondrial pro-apoptotic proteins to the cytosol (e.g. cytochrome c, apoptosis-inducing factor, Smac/DIABLO), and interacting with apoptosis regulatory proteins (e.g. hexokinase and members of the Bcl-2 family35). Purified VDAC1 reconstituted into a planar lipid bilayer shows voltage-dependent bell-shaped conductance. At low membrane potentials (−20 to +20
mV) the channel is at its open state and shows high conductance. At higher positive or negative potentials, the channel conductance is reduced, becoming impermeable to large molecules such as ADP and ATP.35 In the reduced conductance state, the impaired VDAC1 function can lead to an energetic crisis and compromised cell viability.35 Indeed, modulating VDAC1 channel conductance in cancer cells has been proposed as a lead for developing novel anticancer treatments.36
This study demonstrates that in BV-2 microglial cells, CBD affects the homeostasis of cytosolic calcium, mitochondrial function and morphology, and cell viability. These effects seem to arise from a direct CBD-induced inhibition of VDAC1 channel conductance. This idea is supported by the following findings: (1) CBD colocalizes with mitochondrial VDAC1-rich membranes (demonstrated using two fractionation methods coupled to mass spectrometry and western blot analysis). (2) CBD strongly reduces the conductance of purified VDAC1 channels reconstituted into artificial membranes, and (3) CBD directly interacts with fluorescently labeled VDAC1 (shown using the microscale thermophoresis method). This direct interaction of CBD with VDAC1 causing inhibition of channel conductance may be responsible for the strong immunosuppressive and anticancer effects of CBD.
Results
Under serum-free conditions, CBD induced cell death of BV-2 cells as revealed by cell cycle analysis (counts in the subG1). This cell death was highly dependent on CBD concentration (Figure 1a). Cell death was not significantly reversed by the CB2 antagonist SR144528 (SR; Figure 1b). In addition, this effect of CBD was not influenced by pretreatment with pertussis toxin (PTX; Figure 1c), suggesting that it is not acting through a Gi/o coupled GPCR. Furthermore, CBD-induced cell death was not influenced by N-acetylcysteine (NAC) or by the nitric oxide synthase inhibitor L-NAME (Figure 1c).
h treatment under serum-free conditions. Cell death is quantified as the increase in % counts in the subG1 phase (100%=total …CBD was previously suggested to act through the nuclear receptor PPARγ.14 GW9662, an antagonist of this receptor, was recently reported to reduce CBD-induced lung cancer cell death.37 We found that in the BV-2 microglial cell line, GW9662 pretreatment did not reverse the effect of CBD. On the contrary, CBD together with GW9662 enhanced BV-2 cell death (GW; Figure 1b).
Another reported target of CBD is the equilibrative nucleoside transporter 1 affecting the activity at A2A adenosine receptors.12, 38 Indeed, when cells were pre-treated with the specific adenosine antagonist ZM241385, a slight, yet significant, protective effect was observed (ZM; Figure 1b). CBD-induced cell death was also slightly but significantly reduced by ruthenium red (a general TRPV antagonist, as well as inhibitor of calcium-dependent activities, including VDAC1) and by the serotonin 5HT1A antagonist WAY100635 (RR and WAY; Figure 1c). In line with our report regarding the modulation of transcription of cholesterol-related genes such as sterol-O-acyl transferase and CYP27A1 by CBD27 we found that cholesterol enrichment reduced CBD-induced cell death (Figure 1d).
Finally, several groups including McKallip et al.22 Ryan et al.15 and Mato et al.39 reported interactions of CBD with mitochondrial targets as evidenced by its effects on mitochondrial membrane potential, ROS production, and changes in calcium ion homeostasis. We therefore tested the effect of cyclosporin A (Cys A), a blocker of the mitochondrial permeability transition pore (PTP) on CBD-induced cell death. We found that Cys A had a very strong protective effect and almost completely rescued the cells from CBD-induced cell death (Figure 1b).
Next, electron microscopy was used to visualize the cell morphological changes induced by CBD treatment. Cells treated with CBD (2h) under serum-free conditions induced severe mitochondrial swelling (Figure 2, upper panels). A similar trend was observed following 6h of CBD treatment in the presence of 5% serum (data not shown). Vehicle-treated cells showed normal mitochondrial morphology (Figure 2, lower panels).
h of CBD treatment under serum-free conditions. Arrows in the high magnification panel …The mitochondria are an important calcium hub, affecting cytosolic calcium levels. The effect of CBD treatment on cytosolic calcium levels was analyzed using single-cell calcium imaging. Our recordings revealed a quick transient and dose-dependent increase in intracellular calcium, which lasted several minutes (Figures 3a and b). This calcium increase leveled off and was followed by a delayed large increase in calcium level that coincided with mitochondrial swelling and cell death (Figure 3c). The transient intracellular calcium elevation peak was markedly attenuated by removal of extracellular calcium (Figure 3b).
Two functional assays were performed in order to assess changes in mitochondrial functions following CBD treatment: (1) changes in mitochondrial membrane potential (Δψ), and (2) changes in ROS production. These assays revealed that CBD treatment dose-dependently decreases mitochondrial membrane potential with an IC50 of 10μM (Figure 4a) and induces ROS production with an EC50 of 4.9μM (Figure 4b).
μM). Cells were stained with the potentiometric red fluorescent …The possibility of CBD transport into the mitochondria has not been previously investigated. Using two cell fractionation methods coupled to mass spectrometry and western blot analysis, we revealed that following cell pretreatment, CBD colocalized with mitochondrial VDAC1-rich membrane fractions (Figures 5a–c). In the first method according to Wiecowski et al.40 we separated homogenized BV-2 cells into the following organelles: crude mitochondria, lysosomes/plasma membrane, ER, and cytosol. Appropriate antibodies against VDAC1, LAMP-1, β-COP, and β-actin were used as protein markers for the various cell fractions. We found that CBD was present at the highest level in the crude mitochondrial fraction, rich in VDAC1 (Figures 5a and b). To further verify these results, we used an additional fractionation method, which separates cell membrane fractions on a continuous OptiPrep/sucrose density gradient. This method separates membranes on a density gradient with the lighter fractions colocalizing with markers of lipid rafts.33, 41, 42 We found that the distribution of the mitochondrial marker VDAC1, and thus of mitochondrial membranes, was very similar to that of CBD. Both were found predominantly in fraction 6 (Figure 5c).
The effects of CBD on mitochondrial morphology and function together with its colocalization with VDAC1-rich membranes led us to hypothesize an interaction between VDAC1 and CBD. To test the physiological interaction of CBD with VDAC1, purified sheep VDAC1 was reconstituted into a planar lipid bilayer and the effect of CBD on its channel conductance was analyzed. Recordings of the current through the channel before and after the addition of CBD revealed a direct and strong inhibition of VDAC1 conductance by CBD. VDAC1 conductance is voltage-dependent, with the highest conductance obtained between –20 and +20mV, and with decreased conductance at both high negative or positive potentials. At −10mV, when VDAC1 is at its fully open state, CBD strongly decreased VDAC1 conductance (Figure 6ai). Moreover, at −60mV when VDAC1 shows different conducting sub-states, CBD maintained VDAC1 at a low conductance state (Figure 6aii). Overall, in a voltage range between 60 to −60mV, CBD reduced VDAC1 conductance at all applied voltages (Figure 6b). This effect did not depend on the side of CBD addition, the cytosol (cis) side or the intermembrane space (trans) side, suggesting that CBD can reach the VDAC1-binding site regardless of the membrane side of administration.
mV (I) and 0 to −60mV (II), were recorded before (control) …In order to test the physical interaction between CBD and purified VDAC1, we used the microscale thermophoresis method. This method records the directed movement of particles in a microscopic temperature gradient and enables quantitative analysis of molecular interactions in solution. The results show that CBD interacted with purified fluorescently labeled rat VDAC1 with a Kd of 11.2±6μM, whereas N-arachidonoyl ethanolamine (AEA), an endogenous cannabinoid that was used as a control, showed no interaction with fluorescently labeled VDAC1 (Figure 6c).
Discussion
The effects of CBD on microglial cells in vitro and in vivo led us to search for the molecular targets that may be affecting cell function and viability. Using cell cycle analysis, we screened many of the previously reported CBD targets, and identified the involvement of a mitochondrial target. We hypothesized that this target should differ from the previously described mitochondrial sodium–calcium exchanger affected by CBD in neurons and glia.15 This is because CBD in these cells has been reported to have neuroprotective effects against mitochondrial toxins and did not affect cell viability.15, 16 Additionally, the effects of CBD on calcium regulation in neurons could be reversed by the sodium–calcium exchanger inhibitor, CGP 37157, but not by the PTP antagonist Cys A. Conversely, in BV-2 cells Cys A (but not CGP 37157, data not shown) protected against CBD-induced cell death. These results support the idea of a novel mitochondrial target affecting BV-2 microglial cell viability.
Cys A which was found to be protective against CBD-induced cell death is an inhibitor of the mitochondrial PTP, a large pore of unresolved molecular structure that increases the permeability of the inner mitochondrial membrane.43 While transient PTP opening may serve the purpose of providing a fast calcium release mechanism, persistent PTP opening is followed by deregulated release of matrix calcium, loss of mitochondrial membrane potential, mitochondrial swelling and rupture of the OMM.35, 36, 44, 45
Aside from the protective effect of Cys A that points to a cascade involving the PTP, our study supports a specific role for VDAC1 (a proposed constituent of PTP35, 45) in CBD-induced cell death. We show using two fractionation methods that CBD colocalizes with VDAC1-rich mitochondrial membranes. Moreover, using single VDAC1 channel recordings we provide evidence that CBD potently decreases VDAC1 channel conductance, regardless of the side (cis or trans) of CBD addition, suggesting a direct interaction of CBD with VDAC1. This is further supported by our microscale thermophoresis results revealing a direct interaction between CBD and purified VDAC1 with affinity in the range of concentrations producing its cellular effects.
In its open state, VDAC1 is permeable to ions and to uncharged molecules up to 5kDa, thus mediating a flux of ions, nucleotides and other metabolites across the OMM.35 VDAC1 closure results in reduced metabolite exchange across the mitochondrial OMM affecting cellular metabolism and cell viability.46, 47The interaction between CBD and VDAC1 results in reduced channel conductance and eventually leads to cell death. A similar effect on VDAC1 conductance was reported for several anticancer drugs (for review, refer to previous studies35, 36). This observation may explain the antitumorigenic effects of CBD as reported by several groups.3, 9
Our cell cycle analysis showed a strong protective effect of cholesterol enrichment on the viability of BV-2 cells. This may be explained by the described interaction of VDAC1 with cholesterol. Specifically, Popp et al.48 reported that sterols are essential for proper insertion of VDAC1 into bilayer membranes and that cholesterol increases VDAC1 conductance. Based on this, enriching membranes with cholesterol could potentially counteract the CBD-induced reduction in VDAC1 conductance. Recent studies further support a VDAC1–cholesterol interaction. Hiller et al.49 showed that in its 3D structure VDAC1 contains cholesterol molecules that are important for its proper folding. Furthermore, studies using mass spectrometry suggested that VDAC is a necessary component of a protein complex involved in mitochondrial membrane cholesterol distribution, affecting oxidative phosphorylation and induction of apoptosis.50, 51
Mitochondria serve as a cellular calcium hub regulating cytosolic calcium levels. VDAC1 appears to be the main channel transporting calcium across the OMM and its permeability to calcium is affected by the channel’s conductance state.52, 53, 54 Specifically, in its closed state, which is associated with reduced cell viability, VDAC1 shows increased calcium flux from the cytosol into the mitochondria.54 In our cells, CBD induced a quick and transient calcium peak (which was dependent on extracellular calcium) followed by a second prolonged peak resulting in cytosolic calcium overload and cell death. CBD-induced VDAC1 channel closure increases mitochondrial calcium levels, leading to increased mitochondrial permeability, cytosolic calcium overload, loss of mitochondrial membrane potential, increased ROS production, mitochondrial swelling and cell death, a scenario well documented for many cell death inducers. We observed a small protective effect against CBD-induced cell death by pretreatment with ruthenium red. This is in line with the reported interaction of ruthenium red with VDAC1,52 which was shown to protect cells against apoptotic stimuli.55 At this point we cannot determine the contribution of each of the ruthenium red molecular targets (TRPV or VDAC1) to the observed changes in cellular viability.
Unfortunately, we were not able to get efficient siRNA silencing of VDAC1 in BV-2 cells, probably due to the inherent difficulty to transfect BV-2 cells with good efficiency.56 We therefore performed VDAC1 silencing in the human glioma cell line U-87 previously reported to be susceptible to CBD-induced cell death.57 We found that reduction in VDAC1 expression by 70% reduced the susceptibility of these cells to CBD-induced apoptosis. In scrambled siRNA-treated cells, CBD increased apoptosis by 28% over vehicle, while in siVDAC1-treated cells CBD increased apoptosis by only 16% over vehicle, n=3 (data not shown).
In summary, in this study we have identified VDAC1 as a new molecular target for CBD. Our study suggests that CBD-induced cell death may occur through the inhibition of VDAC1 conductance and that this interaction may be responsible for the anticancer and immunosuppressive properties of CBD. However, we cannot rule out the possibility that the interaction of VDAC1 with CBD leads to cell death indirectly, for example by increasing the concentration of CBD in the mitochondria, thus leading to the increased formation of ROS and mitochondrial membrane permeability. We hypothesize that cancer and immune cells are more susceptible to CBD-induced cell death compared with other cell types due to their high proliferative rate, metabolic rate, and VDAC1 activity.
Materials and Methods
Reagents
GW9662 and cyclosporin A (Cys A) (dissolved in DMSO), WAY100635 (diluted in saline), Ruthenium red and Nω-Nitro-l-arginine methyl ester (L-NAME) (diluted in water), and cholesterol (dissolved in sesame oil) were purchased from Sigma (St. Louis, MO, USA). Pertussis toxin (PTX), N-acetyl cysteine (NAC, diluted in water), and ZM241385 (dissolved in DMSO) were purchased from Tocris Bioscience (Bristol, UK). Cannabinoids (dissolved in either ethanol or DMSO) were obtained from NIDA. Other reagents were supplied with respective kits (see Flow cytometry section).
Cell culture
The BV-2 murine microglial cell line, originally generated by Professor E. Blasi (University of Perugia, Perugia, Italy), was kindly provided by Professor EJ Choi from the Korea University (Seoul, Korea). Cells were grown under 5% CO2 at 37°C in a growth medium composed of Dulbecco’s modified Eagle’s medium containing high glucose supplemented with 5% heat-inactivated fetal bovine serum, streptomycin (100μg/ml), and penicillin (100U/ml) (Biological Industries Ltd., Kibbutz Beit Haemek, Israel).
siRNA and cell transfection
Scrambled and human specific hVDAC1-siRNAs were synthesized by Dharmacon (Lafayette, CO). The following sequences were used:
si hVDAC1 sense: 238–5′-ACACUAGGCACCGAGAUUA3′-3′–256 and antisense: 5′-UAAUCUCGGUGCCUAGUGU-3′.
Scrambled sense: 5′-GCAAACAUCCCAGAGGUAU-3′ and antisense: 5′AUACCUCUGGGAUGUUUGC3′.
U-87 cells were seeded (150000cells/well) on six-well culture dishes to 30–50% confluence and transfected with 50nM siRNA hVDAC1 using JetPRIME reagent (PolyPlus transfection, Illkirch, France) according to the manufacturer’s instructions.
Flow cytometry
Cell viability was measured by flow cytometry using propidium iodide (PI) staining. BV-2 cells (9 × 105cells in 10cm plates) were plated in growth medium. On the next day, growth medium was replaced with 5ml serum-free growth medium. The cells were pretreated with the indicated antagonists or vehicle, and then treated with CBD or ethanol (as vehicle at a final concentration of 0.1%) for 2h. The cells were then scraped and spun down for 5min at 2000r.p.m. The pellets were washed twice with Dulbecco’s PBS without Ca2+/Mg2+ and fixed in 70% ethanol at −20°C overnight. Cells were then washed in PBS and incubated with RNaseA (0.2mg/ml) at 37°C, PBS-rinsed and stained with PI (50μg/ml) for 15min on ice. FACS analysis of 20000 cells (for each sample) was performed using a flow cytometer (Becton–Dickinson FACS Calibur, San Jose, CA, USA). The PI emission was detected in the FL2 channel using emission filter of 585nm. The data were analyzed using CellQuest software (BD, Oxford, UK). Apoptotic cells were defined as cells in the subG1 phase with hypodiploid DNA content.
Electron microscopy
BV-2 cells plated at 9 × 105cells per 10cm plate were treated with 10μM CBD or ethanol (as vehicle at a final concentration of 0.1%) for 6h in the presence of 5% serum, or for 2h in the absence of serum. Cells were fixed for 2h in a fixative containing 3% paraformaldehyde, 2%glutaraldehyde, and 5mM CaCl2 in 0.1M cacodylate buffer (pH 7.4). Cells were scraped, pelleted, and embedded in agar noble (in a final concentration of 1.7%) and post-fixed with 1% OsO4, 0.5% potassium dichromate, and 0.5% potassium hexacyanoferrate in 0.1M cacodylate buffer. The pellet was stained en bloc with 2% aqueous uranyl acetate followed by ethanol dehydration and embedding in EMbed812 (Electron microscopic sciences, Fort Washington, PA, USA). Sections were cut using a diamond knife on a Leica Ultracut E ultramicrotome and examined in a JEM 1200EXII transmission electron microscope (JEOL USA Inc., Peabody, MA, USA) equipped with an AMT XR-60 digital camera (Advanced Microscopy Techniques).
Single-cell calcium imaging
BV-2 cells were plated on poly-l-lysine-coated glass coverslips. Twenty four hours later cells were loaded with 3μM Fura-2 AM in phenol red-free DMEM (containing 25mM HEPES) for 60min at room temperature. Cells were then washed three times and mounted onto an inverted microscope (TS-100; Nikon, Tokyo, Japan). Test compounds were added to the cells after 60s baseline recording. During recording, cells were alternately excited at 340 and 380nm using a xenon arc illuminator by switching optical filters mounted in a computer-controlled Sutter wheel (Sutter Instrument Company, Novato, CA, USA). Excitation light was reflected from a dichroic mirror through a × 20 plan fluor objective. Light emitted at an emission wavelength of 510nm was projected onto a Cohu 4920 cooled charge-coupled device camera (Cohu, San Diego, CA, USA), and intracellular calcium concentrations were analyzed with the InCyt Im2 image acquisition and analysis software (Intracellular Imaging Inc., Cincinnati, OH, USA) calibrated using a Fura-2 calibration kit (Invitrogen, Grand Island, NY, USA).
Imaging flow cytometry (IFC)
Cells were stained as described below and 10000 events from each sample were collected and recorded using the ImageStreamX instrument. Samples were analyzed using IDEAS 4.0 software (Amnis Corp., Seattle, WA, USA). Cells were gated for single cells using the area and aspect ratio features, and for focused cells using the Gradient RMS feature, as previously described.58 For ROS detection, the Image-IT LIVE Green ROS Detection Kit was used according to the manufacturer’s instructions (Molecular Probes Inc., Eugene, OR, USA). Briefly, cells were incubated with either CBD (0.1, 1, 5, 10μM), ethanol (as vehicle at 0.1% final concentration), or 300μM tert-butyl hydroperoxide (TBHP) for 30min in phenol red-free DMEM containing 25mM HEPES. Cells were then labeled with 25μM carboxy-H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) for 30min at 37°C in the dark. Hoechst 33342 stain was added during the last 5min of incubation. Cells were washed twice by spin down (1000 × g for 5min) and filtered through a 40-μm mesh before IFC analysis. Data analysis was based on calculating median pixel intensity of gated cells (focused and single). To determine the changes in mitochondrial membrane potential the cells were incubated in phenol red-free DMEM containing 25mM HEPES and treated for 40min at 37°C in the dark with CBD (1, 5, 10, 15μM), ethanol, or 50μM carbonyl cyanide 3-chlorophenyl hydrazone (CCCP). The dye tetramethylrhodamine-methyl ester (TMRM) at 250nM (Santa Cruz Biotechnology, Dallas, TX, USA) was added for the last 20min of incubation. Cells were washed twice by spin down and filtered through a 40-μm mesh before IFC analysis. Image data analysis was based on calculating bright detailed intensity (BDI), defined as the intensity of localized bright spots within the masked area in the image. BDI computes the intensity of bright spots that are three pixels in radius or less. The local background around the spots is removed before the intensity computation.
Antibodies
Coatomer subunit β (β–COP) mouse monoclonal antibody (1:1000; Sigma-Aldrich, St. Louis, MO, USA, #6160), detecting a 110-kDA protein was used with an HRP-goat anti-mouse IgG (1:10000) secondary Ab. VDAC1 rabbit polyclonal antibody (1:1000; Abcam, ab-15895) detecting a 34-kDa protein was used with an HRP-goat anti-rabbit IgG (1:10000). Mouse monoclonal antibody against β-actin (1:10000; Santa Cruz Biotechnology) detecting a 45-KDa protein was used with an HRP-goat anti-mouse IgG (1:10000) secondary Ab. Rat monoclonal anti LAMP1 (1:1000; Developmental studies hybridoma bank, NIH, 1D4B) detecting a 110-KDa protein was used with an HRP-rat anti-mouse IgG (1:10000) secondary Ab.
Western blot analysis
Membrane components present in fractions were lysed by the addition of SDS, NP-40 and Triton X-100 to the final concentrations of 0.5, 1, and 1%, respectively. Samples were incubated on ice for 30min. Laemmli loading buffer was added to the samples and they were denatured for 3min at 70°C. Samples were separated by 10% SDS–PAGE and transferred to nitrocellulose membranes. The membranes were incubated in blocking buffer consisting of 10mM Tris-HCl (pH 7.5), 150mM NaCl, and 0.1% Tween-20 (TBST) with 5% w/v skim milk. The membranes were washed with TBST for five times and incubated with primary antibody in 3% BSA for 1h at room temperature or overnight at 4°C. The membranes were washed five times with TBST, and then incubated for 1h with the appropriate secondary antibodies. The blots were washed five times and processed using chemiluminescence detection.
Cellular organelle fractionation
Cellular fractions of crude mitochondria, lysosomes+plasma membrane, ER, and cytosol were isolated according to Wieckowski et al.40 Briefly, 20 × 150mm cell culture dishes (per condition) were incubated with CBD (10μM, 3h, 5% serum). Medium was removed and cells were washed twice with calcium- and magnesium-free PBS. Cells were scraped in DMEM and collected into 50ml tubes. Cells were centrifuged at 600 × g for 5min a 4°C, resuspended in a small volume of PBS and combined. Centrifugation was repeated (600 × g for 5min at 4°C) and cell pellet was resuspended in 20ml ice-cold IBcells-1 buffer (225mM mannitol, 75mM sucrose, 0.1mM EGTA, and 30mM Tris-HCl, pH 7.4). Cells were then lysed by passage through a 27g X 3′′ needle for 20 times and centrifuged at 600 × g for 5min at 4°C. Supernatant was collected and pellet was discarded. Supernatant was centrifuged again at 600 × g for 5min at 4°C, collected, and centrifuged again at 7000 × g for 10min at 4°C. The supernatant (S1) was collected and saved at 4°C for further separation of cytosolic, lysosomal, and ER fractions. The pellet containing the crude mitochondria was resuspended in IBcells-2 buffer (225mM mannitol, 75mM sucrose, and 30mM Tris-HCl, pH 7.4) and centrifuged at 7000 × g for 10min at 4°C. The supernatant was discarded and the pellet was resuspended in IBcells-2 buffer and centrifuged at 7000 × g for 10min at 4°C. The supernatant was discarded and the pellet was resuspended in IBcells-2 buffer and centrifuged at 10000 × g for 10min at 4°C. The pellet containing crude mitochondria was resuspended in 2ml MRB buffer (250mM mannitol, 5mM HEPES (pH 7.4), and 0.5mM EGTA), and 0.4ml of it was frozen in −20°C for lipid and protein analysis. In order to separate additional organelles, S1 supernatant was centrifuged at 20000 × g for 30min at 4°C. The pellet containing the lysosomal+plasma membrane fraction was frozen at −20°C for lipid and protein analysis. The supernatant was further centrifuged at 100000 × g for 1h at 4°C, yielding an ER fraction (pellet) and a cytosolic fraction (supernatant), which were frozen at −20°C for lipid and protein analysis.
Cell membrane fractionation
Procedures for membrane fractionation were adapted from the method of Macdonald and Pike.41 Briefly, cells were plated 24h before the experiment. On the day of the experiment, cells from four 150mm plates (for each experimental condition) were washed once with PBS and scraped into a total of 25ml of 4°C base buffer (20mM Tris-HCl, 250mM sucrose, pH 7.8) containing 1mM calcium chloride and 1mM magnesium chloride. Cells were centrifuged in a polystyrene tube at 200 × g for 7min at 4°C. The pellet was resuspended in 1.0ml of base buffer containing calcium chloride, magnesium chloride, and protease inhibitor cocktail (Sigma Aldrich, cat # P8340). Cells were then lysed by passage through a 27g X 3′′ needle for 20 times and centrifuged at 1000 × g for 10min at 4°C. The post-nuclear supernatant was transferred to a new tube. The pellet was resuspended in base buffer containing calcium chloride, magnesium chloride, and protease inhibitors, and the procedure was repeated. The two post-nuclear supernatants were combined and 50% OptiPrep (Axis Shield, Dundee, UK) in base buffer was added to give a 4ml solution of 25% OptiPrep. This solution was laid under an 8ml continuous density gradient of 20%–0% OptiPrep in base buffer in Ultra-Clear (14 × 89mm) centrifuge tubes. The gradient was centrifuged using an SW-41 swinging bucket rotor (Beckman Coulter, Fullerton, CA, USA) for 90min at 52000 × g at 4°C. Fractions of 0.75ml were collected from each gradient.
Mass spectrometry
The quantity of CBD in each of the fractions was measured as previously described.33 Briefly, fractions were diluted with 100% HPLC grade methanol. Each sample was spiked with 200pmol of d8-N-arachidonoyl glycine and HPLC grade water was added to make a 75% aqueous solution. These 75%, aqueous solutions were loaded onto 500mg C18 Bond Elut solid phase extraction columns (Agilent, Lake Forest, CA, USA) that were preconditioned with 5ml HPLC grade methanol followed by 2.5ml HPLC water. The columns were then washed with 20ml water. Sequential elutions (1.5ml each of 60, 75, 85, and 100% methanol) were collected for mass spectrometric analysis. An aliquot of each of the eluates was loaded using a Shimadzu 20AC autosampler (Shimadzu, Wilmington, DE, USA) onto a reversed-phase Zorbax 2.1 × 50mm C18 column maintained at 40°C. HPLC gradient formation at a flow rate of 200μl/min was achieved by a system comprising a Shimadzu controller and two Shimadzu LC10ADvp pumps. Lipid levels in the samples were analyzed in multiple reaction monitoring (MRM) mode on a triple quadrupole mass spectrometer, API 3000 (Applied Biosystems/MDS SCIEX, Foster city, CA, USA), with electrospray ionization. Methods for lipid analysis were optimized by flow injection of lipid standards. All quantitation calculations were based on calibration curves using the CBD synthetic standard. The following molecular ion and fragment ion pair was used to quantify CBD in MRM mode: CBD 315.2→193.1.
VDAC1 purification, channel reconstitution and recordings
VDAC1 from sheep liver mitochondria was solubilized with lauryl-dimethylamine-oxide (LDAO) and purified using hydroxyapatite: celite resin as previously described.59 VDAC1 channel was reconstituted into a planar lipid bilayer (PLB) prepared from soybean asolectin dissolved in n-decane (30mg/ml). Purified VDAC1 was added to the cis chamber containing 0.5M NaCl and 10mM HEPES, pH 7.4. After one or more channels were inserted into the PLB, excess protein was removed by prefusing the cischamber to prevent further channel incorporation. Currents were recorded by voltage-clamping using a Bilayer Clamp BC-525B amplifier (Warner Instruments, Hamden, CT, USA). Current was measured with respect to the trans side of the membrane (serving as ground). The current was digitized on-line using a Digidata 1200 interface board and pCLAMP 10.2 software (Axon Instruments, Union City, CA, USA).
Microscale thermophoresis
Microscale thermophoresis (MST) reflects the directed movement of particles in a microscopic temperature gradient, and enables quantitative analysis of molecular interactions in solution at microliter scale. MST analysis was performed using a NanoTemper Monolith NT.115 apparatus, as recently described.60 Briefly, purified rat liver mitochondrial VDAC1 was fluorescently labeled using the NanoTemper Protein-labeling kit Blue (Nano Temper Technologies, Munich, Germany). A concentration of 133nM VDAC1 was incubated for 30–60min at room temperature in the dark with different concentrations of CBD (in PBS containing 2% ethanol as vehicle). The samples (3–5μl) were loaded into standard glass capillaries (Monolith NT Capillaries) and thermophoresis analysis was performed (LED 40%, IR laser 80%). Dissociation constants were calculated using the NanoTemper 1.2.206 analysis program.
Statistical analysis
SPSS was used for statistical analysis. Results were expressed as the mean±S.E.M. and analyzed by one-way analysis of variance (ANOVA) followed by Bonferroni or LSD post hoc tests (P<0.05 has been considered as significant). Dose–response curves were calculated and plotted using GraphPad prism software (La Jolla, CA, USA).
Acknowledgments
This work was supported by the Dr. Miriam and Sheldon G Adelson Medical Research Foundation. AJ is supported by the Israeli Ministry for Absorption in Science. We thank Dr. Or Golan and Professor Asher Shainberg from The Cancer Research Center, Bar Ilan University, Ramat Gan, Israel, for the preliminary experiments on mitochondrial membrane potential.
Glossary
- VDAC1
- voltage-dependent anion channel 1
- CBD
- cannabidiol
- PPARγ
- peroxisome proliferator-activating receptor γ
- TRP
- transient receptor potential channels
- ROS
- reactive oxygen species
- OMM
- mitochondrial outer membrane
- Cys A
- cyclosporin A
- PTX
- pertussis toxin
- TBHP
- tert-butyl hydroperoxide
- MST
- microscale thermophoresis
- PTP
- mitochondrial permeability transition pore
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