A nonpsychoactive cannabinoid cannabidiol (CBD) has been shown to exert potent anti-inflammatory and antioxidant effects and has recently been reported to lower the incidence of diabetes in nonobese diabetic mice and to preserve the blood-retinal barrier in experimental diabetes. In this study we have investigated the effects of CBD on high glucose (HG)-induced, mitochondrial superoxide generation, NF-κB activation, nitrotyrosine formation, inducible nitric oxide synthase (iNOS) and adhesion molecules ICAM-1 and VCAM-1 expression, monocyte-endothelial adhesion, transendothelial migration of monocytes, and disruption of endothelial barrier function in human coronary artery endothelial cells (HCAECs). HG markedly increased mitochondrial superoxide generation (measured by flow cytometry using MitoSOX), NF-κB activation, nitrotyrosine formation, upregulation of iNOS and adhesion molecules ICAM-1 and VCAM-1, transendothelial migration of monocytes, and monocyte-endothelial adhesion in HCAECs. HG also decreased endothelial barrier function measured by increased permeability and diminished expression of vascular endothelial cadherin in HCAECs. Remarkably, all the above mentioned effects of HG were attenuated by CBD pretreatment. Since a disruption of the endothelial function and integrity by HG is a crucial early event underlying the development of various diabetic complications, our results suggest that CBD, which has recently been approved for the treatment of inflammation, pain, and spasticity associated with multiple sclerosis in humans, may have significant therapeutic benefits against diabetic complications and atherosclerosis.

Reagents

1-(2,4-Dichlorophenyl)-5-(4-iodophenyl)-4-methyl-N-4-moepholinyl-1H-pyrazole-3-carboxamide (AM 281) and 6-iodo-2-methyl-1-[2-(4-morpholinyl)ethyl]-1H-indol-3-yl](4-methoxyphenyl) methanone (AM 630) were purchased from Tocris Bioscience (Ellisville, MO). N-piperidino-5-(4-chlorophenyl)-1-(2,4-dichloro-phenyl)-4-methyl-3-pyrazole-carboxamide (SR 141716A; designated as SR1) and {N-[1S]-endo-1,3,3-trimethylbicyclo[2.2.1]heptan-2-yl]-5-(4-chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide} (SR 144528; designated as SR 2) was from Research Triangle Institute (Research Triangle Park, NC). CBD was obtained from either Tocris Bioscience or isolated from hashish as described earlier (15). Sources of all the other reagents used in the experiments are mentioned in the text wherever appropriate.

Cell culture

HCAECs and growth medium were purchased from Cell Applications (San Diego, CA). HCAECs were grown in HCAEC growth medium in cell culture dishes coated with 0.2% gelatin. HCAECs were used for the experiments between passages 3–7. Human monocytic cell line (THP-1) was obtained from American Type Culture Collection and was grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10 mM HEPES, 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin (Invitrogen, CA). The cells were maintained at 37°C in an incubator containing 5% CO2-95% room air.

Cell surface ICAM-1 and VCAM-1 expression assay

Cell surface expression of ICAM-1 and VCAM-1 was measured using in situ ELISA as described (2). In brief, HCAECs were grown in 96-well plates coated with 0.2% gelatin. The cells were treated with either 5 or 30 mM D-glucose (designated as HG) alone for 48 h (33). In some experiments, cells were pretreated with either specific CB1 antagonists SR1/AM 281 or CB2 antagonists SR2/AM 630 for 1 h, followed by continued incubation with HG ± CBD for 48 h.

Western immunoblot analysis of ICAM-1 and VCAM-1 expression

After treatment, cells were washed two times with PBS, and total cell lysate was prepared using radioimmunoprecipitation assay lysis buffer (Pierce Biotechnology) supplemented with cocktail of protease inhibitors (Roche). Protein (25 μg) was resolved in 12% SDS-PAGE and probed with either mouse anti-human ICAM-1/VCAM-1 (1:1,000, R&D systems), and the blots were incubated overnight at 4°C. After being washed, the blots were incubated with secondary antibody goat anti-mouse horseradish peroxidase (Pierce, 1:5,000) for 1 h at room temperature (RT). The blots were then developed with SuperSignal enhanced chemiluminescent substrate solution (Pierce). To verify equal loading, blots were stripped (Pierce) and reprobed with β-actin (Chemicon).

Monocyte adhesion assay

The determination of monocyte adhesion to the endothelial cells was conducted using human THP-1 cells as described (27). In brief, HCAECs were grown to confluence in 24-well plates and treated with HG/CBD ± CB1/CB2 antagonists. THP-1 cells were labeled with 1.5 μM calcein-AM (Molecular Probes; Invitrogen, CA) for 1 h at 37°C in RPMI 1640 containing 1% FBS. Labeled THP-1 cells (105/100 μl) were then added to HCAECs and incubated for 1 h at 37°C in an incubator containing 5% CO2-95% room air. After incubation, the medium containing monocytes was aspirated and the monolayer was gently washed with PBS three times to remove the unbound monocytes. The adherent monocytes were documented using an Olympus IX 81 fluorescent microscope using a × 10 objective with a × 1.5 digital zoom. Three fields were captured per experimental condition. Individual treatments were performed in duplicates, and the entire set of experiments was repeated at least three times. The number of adherent THP-1 cells was counted using National Institutes of Health ImageJ software, and the values were expressed as adhered cells per field.

Monocyte TEM assay

HCAECs were grown to confluence on 0.2% gelatin-coated 3.0-μm polyethylene terepthalate track-etched cell culture inserts (BD Biosciences). The assays were performed essentially as described with minor modifications to the protocol (44). Confluent HCAECs were treated with HG/CDB ± CB1/CB2 antagonists as mentioned in the aforementioned experiments. THP-1 cells were then labeled with 2 μM Cell Tracker Green 5-chloromethylfluorescein diacetate (CMDFA, Molecular Probes) according to the protocol supplied by the manufacturer. Labeled THP-1 cells (3 × 105/200 μl) were added to the upper compartment of the cell culture insert, and the lower compartment of the insert contained 0.5 ml of RPMI 1640. Subsequently, the monocytes were allowed to transmigrate for 4 h at 37°C in an environment of 5% CO2-95% room air. Finally, the culture inserts were removed, and the fluorescence in the lower chamber was measured using the Victor-Wallac Multilabel counter (Perkin Elmer) with excitation at 492 nm and emission at 571 nm. The fluorescence of the lower chamber was proportional to the number of monocytes that were transmigrated. The monocyte TEM was expressed as a percentage, and the set of experiments was repeated independently three times.

Immunofluorescece staining for vascular endothelial cadherin expression

HCAECs were grown to confluence in glass chamber slides (Lab-Tek, Nalgene Nunc) and treated with HG alone or pretreated with CBD for 1 h followed by incubation with HG for 48 h. After the incubation, cells were fixed in 4% paraformaldehyde for 20 min at RT. Subsequently, the cells were permiabilized with 0.2% Triton X-100 for 20 min at RT. After being washed with PBS, the cells were incubated with anti-human vascular endothelial cadherin (VE-cadherin) antibody (1:150; mouse monoclonal, R&D Systems) for 5 h at 4°C. In the subsequent step, the cells were probed with goat anti-mouse FITC conjugate (1:250, Pierce) for 1 h at RT. The nucleus was counterstained with propidium iodide (Molecular Probes). Images were obtained using a fluorescent microscope (Olympus IX 81) at a ×20 objective with a ×1.5 digital zoom.

In vitro vascular permeability assay

HCAECs were grown to confluence in polycarbonate cell culture inserts (0.4 μm pore size, 6.5 mm diameter) in 24-well companion plates (BD Biosciences). The assays were performed as described earlier (48). The growth medium was removed and replaced with 100 μl of 2% FBS growth factor free medium (upper chamber) and 0.5 ml in the lower chamber. Cells were pretreated with either CBD or CB1/CB2 antagonists for 1 h and continued incubation with HG for 48 h. Subsequently, the medium was removed and replaced with 100 μl of serum and growth factor free medium (upper chamber) containing FITC-dextran (40 kDa, Molecular Probes). This step was followed by incubation of the inserts at 37°C in an environment of 5% CO2-95% room air for 45 min. The fluorescence intensities of the solution in the lower chamber were measured using Victor-Wallac Multilabel Counter System (Perkin Elmer) with excitation at 485 nm and emission at 535 nm.

Analysis of NF-κB activation

NF-κB activation was analyzed by measuring inhibitory κB-α factor (IκB-α) degradation and subsequent translocation of NF-κB (p65) to the nucleus using Western blot assays. HCAECs were grown to confluence in 100 mm cell culture dishes. The cells were then treated with either HG or CBD alone for 48 h or pretreated with CB1/CB2 antagonists followed by incubation with HG and/or CBD for 48 h. Subsequently, nuclear and cytoplasmic extracts were prepared using mammalian nuclear protein extraction reagent (NPER; Pierce) by following the manufacturer’s instructions. Nuclear protein (30 μg) or cytoplasmic extracts (20 μg) were resolved on a 12% SDS-PAGE and then transferred to nitrocellulose membrane. After being blocked, the membranes were probed with human anti-IκB-α (rabbit monoclonal, Cell Signaling Technology) in the case of cytoplasmic extract or with anti-human NF-κB (p65) (mouse monoclonal, Invitrogen) in the case of the nuclear extract blots at 1:1,000 and 1:1,500 dilutions, respectively, and incubated overnight at 4°C. Subsequently, the membranes were washed and incubated with appropriate secondary antibodies for 1 h at RT. Finally, the bands were visualized using West Pico chemiluminescence substrate reagent (Pierce). The protein concentration in the cellular extracts was measured using the Bio-Rad Lowry assay kit. The band densities were determined using Image Quant software (Molecular Devices). IκB-α degradation was expressed as the ratio of IκB-α to β-Actin, whereas the NF-κB expression was denoted by percent increase after normalizing the NF-κB expression with histone 2B as the loading control. Furthermore, HG-induced NF-κB activation was also confirmed by using immunoflourescene assay, indicating the nuclear translocation of NF-κB.

Determination of mitochondrial superoxide generation

HCAECs were seeded in 0.2% gelatin-coated 12-well culture dishes and allowed to reach confluence. The cells were then preconditioned in growth factor free medium containing 2% FBS for 4 h, followed by treatments with 5 mM glucose, 30 mM HG, and 30 mM D-mannitol for 48 h. In some experiments, cells were incubated with CBD (4 μM) alone for 48 h, or added 1 h before, the treatment with HG and incubated for 48 h. At the end of incubation, MitoSOX red (5.0 μM; Molecular Probes/Invitrogen) was added to the cells and incubated further for 15 min at 37°C in an atmosphere of 5% CO2-95% room air as described (43, 45). Subsequently, cells were collected by trypsinization and washed in PBS supplemented with 1% BSA, and measurements were performed using FACS caliber system (BD Biosciences). MitoSOX red was excited by laser at 488 nm, and the data were collected at 585/42 nm (FL2) channel. The data were presented by histogram of mean intensity of MitoSOX fluorescence.

iNOS expression and 3-nitrotyrosine accumulation

HCAECs were treated with normal glucose, CBD (4 μM), or HG alone for 48 h or pretreated with CBD for 1 h followed by treatment with HG for 48 h. After incubation, iNOS expression [anti-human iNOS (mouse monoclonal) BD Biosciences] and 3-nitrotyrosine (3-NT) formation (rabbit polyclonal, Cayman Chemicals) were analyzed by Western blot analysis and immunofluorescence staining.

Statistical analysis

All values are represented as means ± SE. Statistical significance of the data was assessed by paired Student’s t-test or one-way ANOVA as appropriate. P < 0.05 was considered significant.

CBD inhibits HG-induced upregulation of ICAM-1 and VCAM-1

HG treatment of HCAECs resulted in a marked upregulation of ICAM-1 (Fig. 1A) and VCAM-1 (Fig. 1B) expression by ~6.0- and 5.5-fold, respectively, when compared with normal glucose-treated cells. This upregulation of ICAM-1 and VCAM-1 expression was dose-dependently reduced by CBD (0–6 μM) pretreatment (Fig. 1, A and B). The effect of CBD was not preventable by either CB1 or CB2 antagonists, which by themselves had no effect on HG-induced increased adhesion molecule expression (Fig. 1, C and D). L-Glucose (30 mM) or D-mannitol (30 mM) had no effect on the upregulation and expression of ICAM-1 and VCAM-1 (data not shown). Similar results were obtained by using Western immunoblot assay (Fig. 1, E and F).

Fig. 1

Effect of cannabidiol (CBD) on high glucose (HG)-induced increased ICAM-1 and VCAM-1 expressions in human coronary artery endothelial cells (HCAECs). Cells were treated with either normal glucose (5 mM) or HG (30 mM) alone for 48 h or pretreated with …

CBD attenuates HG-induced increased monocyte adhesion to the endothelial cells

The representative fields containing monocytes adhered to the endothelial cells with the indicated treatments are shown in Fig. 2A. HG treatment of HCAECs resulted in a ~4.5-fold increase in monocyte adhesion when compared with normal glucose-treated cells (Fig. 2B). Pretreatment of HCAECs with CBD (4 μM) significantly inhibited HG-induced monocyte adhesion to endothelial cells (Fig. 2, A and B), which was not preventable by CB1/CB2 antagonists.

Fig. 2

Effect of CBD on HG-induced increased monocyte adhesion to endothelial cells. A: representative pictures of monocytes adhered to HCAECs. HCAECs were either treated with normal glucose, HG for 48 h, or pretreated with CBD (4 μM) or either CB1 or …

CBD decreases HG-induced monocyte TEM

HG treatment resulted in a marked increase in the monocyte TEM ~3.5 fold when compared with normal glucose-treated cells (Fig. 3). When HCAECs were pretreated with CBD, it significantly reduced the HG-induced increased monocyte TEM, an effect not preventable by either CB1 or CB2antagonists. CB1 or CB2 antagonists had no effect on HG-induced TEM by themselves.

Fig. 3

Effect of CBD on HG-induced increased monocyte transendothelial migration (TEM). HCAECs were treated with either normal glucose, HG, or CBD for 48 h or pretreated with CBD or either CB1 or CB2 antagonists followed by incubation with HG ± CBD and …

CBD attenuates HG-induced disruption of the endothelial barrier function of HCAECs in vitro

HG induced a marked loss of VE-cadherin staining, which was attenuated by CBD pretreatment (Fig. 4A). HG treatment resulted in increased endothelial permeability, characterized by increased seepage of dextran-FITC (40 kDa), which was attenuated by CBD pretreatment, an effect not preventable by either CB1 or CB2 antagonists (Fig. 4B). CB1 or CB2 antagonists had no effect on HG-induced disruption of endothelial barrier function by themselves.

Fig. 4

Effect of CBD on HG-induced barrier dysfunction and mitochondrial superoxide production. A: representative images of immunofluorescence staining for vascular endothelial cadherin expression in HCAECs from 3 independent experiments with similar results. …

CBD moderately attenuates mitochondrial superoxide generation by HG in HCAECs in vitro

HG treatment but not 30 mM D-mannitol (not shown) markedly enhanced mitochondrial superoxide generation (by 3.5-fold) compared with normal glucose in HCAECs. CBD (4 μM) pretreatment attenuated the HG-induced superoxide generation by 23% (Fig. 4C).

CBD attenuates HG-induced increased NF-κB activation in HCAECs

Treatment of HCAECs with HG led to marked degradation of IκB-α (cytoplasm; Fig. 5A) and increased translocation of NF-κB (p65) to the nucleus (Fig. 5, B and C). CBD pretreatment largely prevented NF-κB activation, an effect not preventable by either CB1 or CB2 antagonists.

Fig. 5

Effect of CBD on HG-induced NF-κB activation. A, top: HCAECs were treated as indicated, cytoplasmic extracts were prepared, and Western immunoblot assays were performed to determine inhibitory κB-α factor (IκB-α) …

CBD attenuates HG-induced increased iNOS expression and 3-NT formation

HG treatment of HCAECs resulted in significant increase in the iNOS expression (~3.5 fold) (Fig. 6, A and B) and 3-NT formation (Fig. 6C) when compared with normal glucose-treated cells. All these phenotypic changes induced by HG were inhibited by CBD.

Fig. 6

Effect of CBD on HG-induced inducible nitric oxide (NO) synthase (iNOS) expression and 3-nitrotyrosine (3-NT) formation, proposed protective mechanisms of CBD. A: HCAECs were treated with either normal glucose, CBD (4 μM), HG alone for 48 h or …

We are indebted to Prof. Raphael Mechoulam for providing cannabidiol and valuable comments on the manuscript.

GRANTS

This study was supported by the National Institute on Alcohol Abuse and Alcoholism Intramural Research Program (to P. Pacher).