Cell Immunol. 2016 Nov 9. pii: S0008-8749(16)30135-6. doi: 10.1016/j.cellimm.2016.11.006. [Epub ahead of print]
Cannabidiol (CBD) induces functional Tregs in response to low-level T cell activation.
Abstract
Many effects of the non-psychoactive cannabinoid, cannabidiol (CBD), have been described in immune responses induced by strong immunological stimuli. It has also been shown that CBD enhances IL-2 production in response to low-level T cell stimulation. Since IL-2, in combination with TGF-β1, are critical for Treg induction, we hypothesized that CBD would induce CD4+CD25+FOXP3+Tregs in response to low-level stimulation. Low-level T cell stimulation conditions were established based on minimal CD25 expression in CD4+ cells using suboptimal PMA/Io (4nM/0.05μM, S/o), ultrasuboptimal PMA/Io (1nM/0.0125μM, Us/o) or soluble anti-CD3/28 (400-800ng each, s3/28). CBD increased CD25+FOXP3+ cells from CD4+, CD4+CD25+, and CD4+CD25- T cells, as well as in CD4+ T cells derived from FOXP3-GFP mice. Most importantly, the Us/o+CBD-induced CD4+CD25+ Tregs robustly suppressed responder T cell proliferation, demonstrating that the mechanism by which CBD is immunosuppressive under low-level T cell stimulation involves induction of functional Tregs.
Copyright © 2016 Elsevier Inc. All rights reserved.
KEYWORDS:
CBD; CD4(+)CD25(+)FOXP3(+) Tregs; Immunosuppression
- PMID: 27865421
- DOI: 10.1016/j.cellimm.2016.11.006
- [PubMed – as supplied by publisher]
