Canna~Fangled Abstracts

Effect of metal ions on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp. 99-125.

By March 28, 2018No Comments
Int J Biol Macromol. 2018 Mar 28. pii: S0141-8130(18)30135-1. doi: 10.1016/j.ijbiomac.2018.03.168. [Epub ahead of print]

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Highlights

•The spectroscopy of the interaction of metal ions and lipase had been analyzed.
•Metal ions could improve the thermal stability of lipase.
•Metal ions could improve the hydrolysis rate of lipase Candida sp. 99–125.
•Metal ions might change the selectivity of lipase Candida sp. 99–125.
•Fe3+ was the most suitable metal ion for enhancement of lipase activity.

Abstract

PM 2 site 207In order to study the effect of metal ions on the enzymatic hydrolysis of hemp seed oil by Candida sp. 99-125, the spectroscopy, stability and hydrolytic activity of the biocatalyst were investigated in presence of Ca2+, Mg2+, Fe2+, Fe3+, Cu2+, Sn2+, Pb2+, Zn2+ and Ba2+ metal ions, respectively. The UV spectroscopy showed that all the metal ions enhanced the absorbance but the decrease of fluorescence intensity was observed. All the metal ions could improve the lipase thermal stability except Cu2+ and Ba2+. Hydrolysis of hemp seed oil proved that Ca2+, Fe3+, Pb2+ and Ba2+could significantly improve the hydrolytic rate, and metal ions could influence lipase selectivity. The study revealed that metal ions could improve lipase stability, hydrolysis activity in the hydrolytic process of hemp seed oil by Candida sp. 99-125.


 

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KEYWORDS:

Candida sp. 99–25; Hemp seed oil; Metal ions

PMID: 29604356

 

DOI: 10.1016/j.ijbiomac.2018.03.168

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