Canna~Fangled Abstracts

Induction of Endocannabinoid Levels in Juvenile Rat Brain Following Developmental Chlorpyrifos Exposure.

By June 14, 2013 No Comments
pm1[Epub ahead of print]

Induction of Endocannabinoid Levels in Juvenile Rat Brain Following Developmental Chlorpyrifos Exposure.

Source

Center for Environmental Health Sciences, Department of Basic Sciences, College of Veterinary Medicine Mississippi State University Mississippi State, MS 39762.

Abstract

The endogenous cannabinoids 2-arachidonoylglycerol (2-AG) and anandamide (AEA) play vital roles during nervous system development. The degradation of 2-AG and AEA is mediated by monoacylglycerol lipase (MAGL) and fatty acid amide hydrolase (FAAH), respectively. These enzymes are inhibited following developmental chlorpyrifos (CPF) exposure. To investigate whether this inhibition is persistent or whether accumulation of endocannabinoids in the brain occurs, 10 day old rat pups were orally exposed daily for 7 days to either corn oil or increasing dosages of CPF (1, 2.5, or 5 mg/kg) and forebrains were collected at 4, 12, 24, and 48 h following the last administration. All dosages inhibited cholinesterase (ChE), FAAH, and MAGL, and elevated AEA and 2-AG levels with the greatest effect occurring at 12 h with ChE, FAAH, AEA and 2-AG; 4 h with MAGL. With the high dosage, return to control levels occurred with 2-AG (48 h) only. With the medium dosage, return to control levels occurred with MAGL, 2-AG, and AEA (48 h) but not ChE or FAAH. With the low dosage, return to control levels occurred with MAGL (12 h), ChE and 2-AG (24 h), and AEA (48 h) but not with FAAH. With the lowest dosage, peak inhibition of FAAH (52%) is greater than that of ChE (24%) and that level of FAAH inhibition is sufficient to induce a persistent pattern of elevated AEA. It is possible that this pattern of elevation could alter the appropriate development of neuronal brain circuits.
PMID:

 

23761300

 

[PubMed – as supplied by publisher]
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http://www.ncbi.nlm.nih.gov/pubmed/23761300
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