Spectrochim Acta A Mol Biomol Spectrosc. 2017 Apr 1;182:168-174. doi: 10.1016/j.saa.2017.03.072.
[Epub ahead of print]
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Highlights
- •Extraction of the hemp essential oil and its fractionation.
- •Raman spectral analysis of all the samples.
- •Raman spectral characterization to determine the biochemical composition of the essential oil and different fractions.
- •Principal Component Analysis has differentiated different samples on the basis of their Raman spectral profile.
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Abstract
In this study, Raman spectroscopy along with Principal Component Analysis (PCA) is used for the characterization of pure essential oil(pure EO) isolated from the leaves of the Hemp (Cannabis sativa L.,) as well as its different fractions obtained by fractional distillation process. Raman spectra of pure Hemp essential oil and its different fractions show characteristic key bands of main volatile terpenes and terpenoids, which significantly differentiate them from each other. These bands provide information about the chemical composition of sample under investigation and hence can be used as Raman spectral markers for the qualitative monitoring of the pure EO and different fractions containing different active compounds. PCA differentiates the Raman spectral data into different clusters and loadings of the PCA further confirm the biological origin of the different fractions of the essential oil.
Copyright © 2017 Elsevier B.V. All rights reserved.
KEYWORDS:
Essential oil; Fractional distillation; Hemp; Principal component analysis; Raman spectroscopy
- PMID: 28431313
- DOI: 10.1016/j.saa.2017.03.072
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