The 2-arachidonoylglycerol effect on myosin light chain phosphorylation in human platelets.
Source
Department of Pharmacy, Biochemistry Lab, University of Genoa, Viale Benedetto XV 3, 16132 Genova, Italy.
Abstract
In human platelets the endocannabinoid 2-arachidonoylglycerol (2-AG) stimulates some important pathways leading to thromboxane B2 formation, calcium intracellular elevation, ATP secretion and actin polymerisation. The aim of the present study was to examine the 2-AG effect on myosin light chain (MLC) phosphorylation and to investigate the mechanisms involved. We demonstrated that 2-AG induced a rapid MLC phosphorylation, stimulating both the RhoA kinase (ROCK) and MLC kinase (MLCK) in a dose and time-dependent manner. In addition MLC phosphorylation was strengthened through the MLC phosphatase inhibition. MLC phosphatase inhibition was accomplished through the RhoA/ROCK and protein kinase C mediated phosphorylation of MLC phosphatase inhibiting subunits MYPT1 and CPI-17. The presence of CB1 receptor in human platelets and the involvement of CB1 receptor in MLC phosphorylation and MLC phosphatase inhibition was shown.
Copyright © 2013. Published by Elsevier Masson SAS.
Copyright © 2013. Published by Elsevier Masson SAS.
- PMID:
- 23702422
- [PubMed – as supplied by publisher]
- http://www.ncbi.nlm.nih.gov/pubmed/23702422
