2014 Sep 5. pii: S0303-7207(14)00276-7. doi: 10.1016/j.mce.2014.09.005. [Epub ahead of print]
2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization: influence of endocannabinoid signalling in placental development.
Abstract
A balanced cytotrophoblast cell turnover is crucial for placental development and anomalies in this process associated with gestational diseases. The Endocannabinoid System (ECS) has emerged as a new player in several biological processes. However, its influence during placental development is still unknown. We report here the expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) main metabolic enzymes in human cytotrophoblasts and syncytiotrophoblast. We also showed that 2-AG induced a decrease in placental alkaline phosphatase activity, human chorionic gonadotropin secretion and Leptin mRNA levels. Moreover, 2-AG reduced glial cell missing 1 and syncytin-2 transcription and the number of nuclei in syncytium. These effects were mediated by cannabinoid receptors and may result from 2-AG inhibition of the cAMP/PKA signalling pathway. Our data suggest that 2-AG may interfere with the biochemical and morphological differentiation of human cytotrophoblasts, through a CB receptor-dependent mechanism, shedding light on a role for the ECS in placental development.
Copyright © 2014. Published by Elsevier Ireland Ltd.
Copyright © 2014. Published by Elsevier Ireland Ltd.
KEYWORDS:
2-arachinoylglycerol; endocannabinoid signalling; human cytotrophoblasts; placenta; syncytialization
- PMID:
25199616
[PubMed – as supplied by publisher]
