Effect of metal ions on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp. 99-125.

Int J Biol Macromol. 2018 Mar 28. pii: S0141-8130(18)30135-1. doi: 10.1016/j.ijbiomac.2018.03.168. [Epub ahead of print]

Lu J¹, Wang P², Ke Z², Liu X², Kang Q², Hao L³.

Author information

Highlights

•The spectroscopy of the interaction of metal ions and lipase had been analyzed.
•Metal ions could improve the thermal stability of lipase.
•Metal ions could improve the hydrolysis rate of lipase Candida sp. 99–125.
•Metal ions might change the selectivity of lipase Candida sp. 99–125.
•Fe³⁺ was the most suitable metal ion for enhancement of lipase activity.

Abstract

In order to study the effect of metal ions on the enzymatic hydrolysis of hemp seed oil by Candida sp. 99-125, the spectroscopy, stability and hydrolytic activity of the biocatalyst were investigated in presence of Ca²⁺, Mg²⁺, Fe²⁺, Fe³⁺, Cu²⁺, Sn²⁺, Pb²⁺, Zn²⁺ and Ba²⁺ metal ions, respectively. The UV spectroscopy showed that all the metal ions enhanced the absorbance but the decrease of fluorescence intensity was observed. All the metal ions could improve the lipase thermal stability except Cu²⁺ and Ba²⁺. Hydrolysis of hemp seed oil proved that Ca²⁺, Fe³⁺, Pb²⁺ and Ba²⁺could significantly improve the hydrolytic rate, and metal ions could influence lipase selectivity. The study revealed that metal ions could improve lipase stability, hydrolysis activity in the hydrolytic process of hemp seed oil by Candida sp. 99-125.