Investigation of suitable seed sizes, segregation of ripe seeds, and improved methods of germination for commercial production of hemp (Cannabis sativa L.) sprouts.

BACKGROUND:

With a growing market for functional foods, the nutraceutical properties of hemp sprouts have been investigated in recent studies; however, commercial mass production methods have yet to be developed. This study aimed to identify seed sizes suitable for segregating ripe seeds, which would improve the low germination ratio in high seed density used in commercial hemp sprout production.

RESULTS:

Seeds in the size range of 2.80-3.30 ㎜ collected by sieving, were the most suitable for sprouting based on the distribution ratio (74.9%) and germination ratio (70.0%) in low seed density (0.016 grains ㎜^-2 ). Seed segregation by sinking in 70% ethanol after 2+ h of water infiltration generated high germination ratios of 86.3-94.3% at low seed density, compared to the 64.0% germination ratio in non-segregated seeds. The hemp seed germination ratio geometrically decreased with increasing sowing density. The germination ratio in high seed density (0.29 grains ㎜^-2 ) was increased from 19.9% in seeds only germinating to 58.7% when mixed with sand in a 3:1 ratio. Sprouting yield was significantly increased from 1.64 kg kg^-1 in seeds only germinating to 9.55 kg kg^-1 in seeds germinating when mixed with sand. Delta-9-tetrahydrocannabinol was not detected in hemp sprout.

CONCLUSION:

Hemp sprouts production method may be improved by identifying suitable seed size, segregating ripe seeds and effect of germinating seeds mixed with sand. This can be used in commercial production of hemp sprout, which can be consumed without concern for hallucinogenic effects. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.