2008 May;8(5):732-40. doi: 10.1016/j.intimp.2008.01.018. Epub 2008 Feb 14.
A comparative study on cannabidiol-induced apoptosis in murine thymocytes and EL-4 thymoma cells.
It has been shown that leukemia and glioma cells are sensitive to cannabidiol (CBD)-induced apoptosis, whereas primary monocytes and glia cells are relatively insensitive. In the current study, the cellular events and sensitivity to CBD-induced apoptosis between murine thymocytes and EL-4 thymoma cells were compared. Cannabidiol markedly induced apoptosis in a time- and concentration-related manner in both cells. The efficacy of CBD to induce apoptosis was comparable between the 2 types of T cells, whereas CBD induced apoptosis in thymocytes with a slightly greater potency than in EL4 cells. Time-course analyses revealed CBD-mediated apoptosis occurred earlier in EL-4 cells than that in thymocytes. An increased level of cellular reactive oxygen species (ROS) was detected in both cells with the peak response at 2 h post CBD treatment. Concordantly, CBD triggered a gradual diminishment in the cellular thiols. The presence of N-acetyl-L-cysteine (NAC), a precursor of glutathione, markedly attenuated the induction of apoptosis, and restored the diminished levels of cellular thiols. The results demonstrated that both thymocytes and EL-4 thymoma cells were susceptible to CBD-induced apoptosis and that ROS played a critical role in the apoptosis induction.
- [PubMed – indexed for MEDLINE]
- Apoptosis/drug effects*
- Cell Cycle/drug effects
- Cell Line, Tumor
- Flow Cytometry
- Fluorescent Dyes
- In Situ Nick-End Labeling
- Mice, Inbred BALB C
- Oxidative Stress/drug effects
- Reactive Oxygen Species/metabolism
- Sulfhydryl Compounds/metabolism
- T-Lymphocytes/drug effects*
- Thymus Neoplasms/metabolism
- Thymus Neoplasms/pathology*