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Canna~Fangled Abstracts

Analytical Methodologies for Lipidomics in Hemp Plant

By May 6, 2021May 9th, 2021No Comments
. 2021;2306:257-273.

doi: 10.1007/978-1-0716-1410-5_17.

Andrea Cerrato 1, Anna Laura Capriotti 1, Carmela Maria Montone 2, Sara Elsa Aita 1, Giuseppe Cannazza 3 4, Cinzia Citti 3 4, Susy Piovesana 1, Laganà Aldo 1 4
Affiliations 

Abstract

The chemical composition of Cannabis sativa L. has been extensively studied for tens of years, but little is known about its lipidome. This chapter describes an analytical workflow for polar lipid determination in hemp. After extraction, lipids are enriched and isolated by graphitized carbon black sorbent, and the isolated lipid is analyzed by liquid chromatography (LC) coupled with high resolution mass spectrometry, leading to identification of many lipid species. We have developed a semi-automated platform using commercially available Lipostar software for lipid identification. Our approach affords the identification of 189 polar lipids in hemp extract, including sulfolipids and phospholipids. The number of the identified lipid species is by far the highest ever reported for Cannabis sativa.

 

Keywords: Cannabis sativa, Graphitized carbon black, Lipidomics, Lipostar, Phospholipids, Sulfolipids

References

    1. Russo EB, Jiang H-E, Li X, Sutton A, Carboni A, del Bianco F, Mandolino G, Potter DJ, Zhao Y-X, Bera S, Zhang Y-B, Lü E-G, Ferguson DK, Hueber F, Zhao L-C, Liu C-J, Wang Y-F, Li C-S (2008) Phytochemical and genetic analyses of ancient cannabis from Central Asia. J Exp Bot 59:4171–4182. https://doi.org/10.1093/jxb/ern260 – DOI – PubMed – PMC
    1. Skoglund G, Nockert M, Holst B (2013) Viking and early middle ages northern Scandinavian textiles proven to be made with hemp. Sci Rep 3:2686. https://doi.org/10.1038/srep02686 – DOI – PubMed – PMC
    1. Small E, Beckstead HD (1973) Common cannabinoid phenotypes in 350 stocks of Cannabis. Lloydia 36:144–165. http://www.ncbi.nlm.nih.gov/pubmed/4744553 – PubMed
    1. Blake A, Nahtigal I (2019) The evolving landscape of cannabis edibles. Curr Opin Food Sci 28:25–31. https://doi.org/10.1016/j.cofs.2019.03.009 – DOI
    1. ElSohly MA, Slade D (2005) Chemical constituents of marijuana: the complex mixture of natural cannabinoids. Life Sci 78:539–548. https://doi.org/10.1016/j.lfs.2005.09.011 – DOI – PubMed

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