Skip to main content
Canna~Fangled Abstracts

Bioactive polyphenols separated from hemp seed shells ameliorate H2 O2 -induced oxidative stress injury in human umbilical vein endothelial cells

By January 12, 2023October 12th, 2023No Comments

. 2023 Jan;88(1):537-551.

doi: 10.1111/1750-3841.16424. Epub 2022 Dec 16.

Peng-Wei Xu 1 2, Xiao-Fan Yuan 1, Bing Zhao 1
Affiliations 

Abstract

In the present study, we investigated the protection of hemp seed polyphenols on human umbilical vein endothelial cells (HUVEC) from H2 O2 -mediated oxidative stress injury. Fractions with different polarities were obtained by separating the hemp seed extract using HPD300 macroporous resin-packed column. The fraction, desorbed by 50% ethanol, is rich in polyphenol (789.51 ± 21.92 mg GAE/g) and has the highest antioxidant activity in vitro. HPLC-QTOF-MS/MS identified the main polyphenol components in hemp seed shells: 4 hydroxycinnamic acid amides and 15 lignanamides. The protective effects of hemp seed polyphenol against oxidative-stress injury in HUVEC cells were evaluated by cell viability, intracellular antioxidant parameters, and cell apoptosis assay. After HUVEC cells were precultured with 50 µg/ml hemp seed polyphenols, the cell viability increased significantly from 53.07 ± 2.46% (model group) to 80.65 ± 1.32% (p < 0.01). In addition, the pretreatment of HUVEC cells with polyphenol could substantially increase their intracellular superoxide dismutase activity and reduce their intracellular reactive oxygen species level, malondialdehyde content, and lactate dehydrogenase leakage index. These findings demonstrate the defensive potential of hemp seed polyphenol in reducing the incidence of cardiovascular disease. PRACTICAL APPLICATION: Hemp seed shell waste is produced while producing hemp seed kernel and has abundant phenolic compounds. This research showed that hemp seed polyphenol has potent antioxidant activity in vitro and protects HUVEC cells against H2 O2 -induced oxidative stress injury, suggesting that hemp seed polyphenol has the defensive potential to reduce the incidence of cardiovascular disease. These results indicated that polyphenol separated from hemp seed shells is valuable for further research and development, which will improve the utilization rate of hemp seed.

Keywords: antioxidant, hemp seed, human umbilical vein endothelial cells, oxidative stress, polyphenol

PubMed Disclaimer

Similar articles

See all similar articles

References

REFERENCES

    1. Arts, M., Dallinga, J. S., Voss, H. P., Haenen, G., & Bast, A. (2004). A new approach to assess the total antioxidant capacity using the TEAC assay. Food Chemistry, 88(4), 567-570. https://doi.org/10.1016/j.foodchem.2004.02.008
    1. Bhullar, K. S., & Rupasinghe, H. P. V. (2015). Antioxidant and cytoprotective properties of partridgeberry polyphenols. Food Chemistry, 168, 595-605. https://doi.org/10.1016/j.foodchem.2014.07.103
    1. Chen, T., He, J., Zhang, J., Li, X., Zhang, H., Hao, J., & Li, L. (2012). The isolation and identification of two compounds with predominant radical scavenging activity in hempseed (seed of Cannabis sativa L.). Food Chemistry, 134(2), 1030-1037. https://doi.org/10.1016/j.foodchem.2012.03.009
    1. Chen, T. P., Hao, J. X., He, J. F., Zhang, J. C., Li, Y. C., Liu, R., & Li, L. T. (2013). Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells. Food Chemistry, 138(2-3), 1034-1041. https://doi.org/10.1016/j.foodchem.2012.11.102
    1. Chen, T. P., He, J. F., Zhang, J. C., Li, X. H., Zhang, H., Hao, J. X., & Li, L. T. (2012). The isolation and identification of two compounds with predominant radical scavenging activity in hempseed (seed of Cannabis sativa L.). Food Chemistry, 134(2), 1030-1037. https://doi.org/10.1016/j.foodchem.2012.03.009

MeSH terms

Substances

LinkOut – more resources

  • Full Text Sources

Leave a Reply