First report of Meloidogyne incognita infecting Cannabis sativa in Alabama

doi: 10.21307/jofnem-2021-052. eCollection 2021.

Bisho R Lawaju¹, William Groover², Jessica Kelton³, Kassie Conner¹, Edward Sikora¹, Kathy S Lawrence¹

Affiliations

PMID: 33959722
PMCID: PMC8098102
DOI: 10.21307/jofnem-2021-052

Abstract

Hemp (Cannabis sativa L.) is a new crop in Alabama. In 2019, symptomatic plants with stunted growth, poor root development, and numerous galls were observed in hemp plants grown in Geneva County, AL. After harvest, soil samples were collected from areas with the symptomatic plants and root-knot nematode (Meloidogyne spp.) were found in the soil. Based on morphological features and the polymerase chain reactions using species-specific primers, it was identified as Meloidogyne incognita. Further, a host differential test in a greenhouse assay confirmed it to be M. incognita race 3. The pathogenicity of the nematode to the hemp was confirmed by a modified version of Koch’s postulates. To our knowledge, this is the first report of M. incognita infecting Cannabis sativa in Alabama.

Keywords: Alabama, Cannabis sativa, Meloidogyne incognita, hemp, identification, root-knot nematode

References

1. Coburn, J. and Desaeger, J. . 2020. Root-knot nematode host status and susceptibility of different hemp cultivars. Poster presented at Entomological Society of America Virtual Annual Meeting; November 16–19, 2020.
1. Groover, W. , Lawrence, K. and Donald, P. . 2019. Reproductive rate differences of root-knot nematodes from multiple crops in a single field. Nematropica 49: 152–156.
1. Hansen, Z. , Gwinn, K. , Hale, F. , Kelly, H. and Stewart, S. . 2020. Hemp disease and pest management. W 916, UT Extension, Institute of Agriculture, University of Tennessee).
1. Hartman, K. M. and Sasser, J. N. . 1985. “Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology”, In Barker, K. R. , Carter, D. D. and Sasser, J. N. (Eds), An Advanced Treatise on Meloidogyne vol. II, Methodology, Vol. 2 North Carolina State University Graphics, Raleigh, pp. 69–77.
1. Hunt, D. J. and Handoo, Z. A. . 2009. “Taxonomy, identification and principal species”, In Perry, R. N. and Moens and Star, J. L. (Eds), Root-knot nematodes Vol. 1, CAB International, pp. 55–88..