Gamma-irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells.
Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 microg/ml CBD and 15 microg/ml CBD-DMH, respectively, after a 24 h treatment. Prior exposure of the cells to gamma-irradiation (800 cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or gamma-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.
- [PubMed – indexed for MEDLINE]
- Apoptosis/drug effects*
- Apoptosis/radiation effects*
- Cannabidiol/analogs & derivatives*
- Caspase 3
- Enzyme Activation/drug effects*
- Enzyme Activation/radiation effects*
- Gamma Rays
- HL-60 Cells/drug effects
- HL-60 Cells/radiation effects
- Monocytes/drug effects
- Monocytes/radiation effects