Abstract
BACKGROUND:
Sodium lauryl sulfate (SLS) and ultraviolet radiation (UVR) represent two commonly-encountered cutaneous inflammatory stimuli. Differing histopathological and clinical features implicate involvement of alternative inflammatory pathways; bioactive lipid mediators, including eicosanoids, endocannabinoids and sphingolipids, are likely candidates for regulation of the divergent inflammatory responses.
OBJECTIVES:
Perform a comprehensive assessment of bioactive lipid involvement in SLS- and UVR-induced inflammatory responses, to provide a better understanding of bioactive lipid mediator pathways in irritant inflammation.
METHODS:
Buttock skin in 10 healthy volunteers was treated with two minimal erythema doses of UVR (275-380nm, peak 305nm), or an SLS dose optimised for each individual to produce a comparable, moderate erythema. Punch biopsies were taken 24h post-challenge and from untreated skin, and separated into dermis and epidermis. Lipids (including 15 prostanoids, 15 hydroxy fatty acids (HFA), 9 endocannabinoids and related N-acyl ethanolamides (NAE), and 21 sphingolipids) were extracted and quantified using liquid chromatography coupled to tandem mass spectrometry.
RESULTS:
We observed increased epidermal NAE and HFA expression in response to SLS, but not the UVR-induced low level inflammation. Significant changes following SLS treatment included augmented levels of NAE, possessing pro-inflammatory and some reported anti-inflammatory properties, with 3.7-fold (P=0.025) and 3-fold (P=0.009) expression of palmitoyl and stearoyl ethanolamides, respectively, in addition to 1.9-fold (P=0.017) expression of the chemoattractant 12- hydroxyeicosatetraenoic acid (12-HETE).
CONCLUSION:
The differential bioactive lipid upregulation implicates their involvement in skin irritant responses, potentially reflecting roles in inflammatory cell recruitment and subsequent resolution of inflammation and giving scope for new treatment approaches in irritant dermatitis. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
- PMID:
- 26947140
- [PubMed – as supplied by publisher]
