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Canna~Fangled Abstracts

Raman spectroscopy enables highly accurate differentiation between young male and female hemp plants

By March 14, 2022No Comments

. 2022 Mar 13;255(4):85.

doi: 10.1007/s00425-022-03865-8.

Samantha Higgins 1, Russell Jessup 2, Dmitry Kurouski 3
Affiliations 

Abstract

Hand-held Raman spectroscopy can be used for highly accurate differentiation between young male and female hemp plants. This differentiation is based on significantly different concentration of lutein in these plants. Last year, a global market of only industrial hemp attained the value of USD 4.7 billion. It is by far the fastest growing market with projected growth of 22.5% between 2021 and 2026. Hemp (Cannabis sativa L.) is a dioecious species that has separate male and female plants. In hemp farming, female plants are strongly preferred because male plants do not produce sufficient amount of cannabinoids. Male plants are also eliminated to minimize a possibility of uncontrolled cross-fertilization of plants. Silver treatments can induce development of male flowers on genetically female plants in order to produce feminized seed. Resulting cannabinoid hemp production fields should contain 100% female plants. However, any unintended pollination from male plants can produce unwanted males in production fields. Therefore, there is a growing demand for a label-free, non-invasive, and confirmatory approach that can be used to differentiate between male and female plants before flowering. In this study, we examined the extent to which Raman spectroscopy, an emerging optical technique, can be used for the accurate differentiation between young male and female hemp plants. Our findings show that Raman spectroscopy enables differentiation between male and female plants with 90% and 94% accuracy on the level of young and mature plants, respectively. Such analysis is entirely non-invasive and non-destructive to plants and can be performed in seconds using a hand-held spectrometer. High-performance liquid chromatography (HPLC) analysis and collected Raman spectra demonstrate that this spectroscopic differentiation is based on significantly different concentrations of carotenoids in male vs female plants. These findings open up a new avenue for quality control of plants grown in both field and a greenhouse.

Keywords: Chemometrics; HPLC; Hemp; Lutein; Raman spectroscopy; Sex determination.

References

    1. Adar F (2017) Carotenoids—their resonance Raman spectra and how they can be helpful in characterizing a number of biological systems. Spectroscopy 32(6):12–20
    1. Agarwal UP (2006) Raman imaging to investigate ultrastructure and composition of plant cell walls: distribution of lignin and cellulose in black spruce wood (Picea mariana). Planta 224(5):1141–1153. https://doi.org/10.1007/s00425-006-0295-z – DOI – PubMed
    1. Almeida MR, Alves RS, Nascimbem LB, Stephani R, Poppi RJ, de Oliveira LF (2010) Determination of amylose content in starch using Raman spectroscopy and multivariate calibration analysis. Anal Bioanal Chem 397(7):2693–2701. https://doi.org/10.1007/s00216-010-3566-2 – DOI – PubMed
    1. Appendino G, Gibbons S, Giana A, Pagani A, Grassi G, Stavri M, Smith E, Rahman MM (2008) Antibacterial cannabinoids from Cannabis sativa: a structure-activity study. J Nat Prod 71(8):1427–1430. https://doi.org/10.1021/np8002673 – DOI – PubMed
    1. Borrelli F, Fasolino I, Romano B, Capasso R, Maiello F, Coppola D, Orlando P, Battista G, Pagano E, Di Marzo V, Izzo AA (2013) Beneficial effect of the non-psychotropic plant cannabinoid cannabigerol on experimental inflammatory bowel disease. Biochem Pharmacol 85(9):1306–1316. https://doi.org/10.1016/j.bcp.2013.01.017 – DOI – PubMed

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