Scent Knows Better: Utilizing Volatile Organic Compounds as a Robust Tool for Identifying Higher Cannabidiol- and Tetrahydrocannabinol-Containing Cannabis Cultivars in Field Conditions

The primary cannabinoids cannabidiol (CBD) and tetrahydrocannabinol (THC), found in cannabis, are known to originate from genetic diversity, resulting in distinct characteristics. This study aimed to identify VOC markers to distinguish between higher CBD and THC cannabis cultivars under field conditions. Among the 58 VOCs, β-caryophyllene and α-humulene were primary VOCs across all cannabis cultivars. Intriguingly, certain terpene VOCs exhibited contrasting trends between higher CBD and higher THC cannabis cultivars. Eudesma-3,7(11)-diene and α-guaiol consistently appeared as highlighted compounds, suggesting their potential to distinguish between higher CBD and THC cannabis cultivars. ROC curve analysis revealed approximately 94% predictive accuracy for these putative markers. Given the current focus on VOCs as sensor markers for plant health, growth, and quality, the identified VOC markers─applicable across varieties and growth stages─could enable nondestructive, rapid, and accurate identification of CBD- and THC-rich cannabis species in field conditions.