OBJECTIVE:

To select the optimum extracting procedure for cannabinoids from hemp seed oil.

METHODS:

The optimum extracting procedure was selected with the content of cannabinol and delta9-tetrehydrocannabinol from hemp seed oil by orthogonal test design. We have examined three factors that may influence the extraction rate: the time of extraction, the times of extraction and the amount of methanol.

RESULTS:

The optimum extraction condition was adding 5 ml, two times amount of methanol into hemp seed oil for 15 min.

CONCLUSION:

The above extraction process gave the most rational, stable, feasible and satisfactory results. The method is convenient.