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Canna~Fangled Abstracts

The Fatty Acid Amide Hydrolase in Lymphocytes from Sedentary and Active Subjects.

By June 26, 2013No Comments
 pm2[Epub ahead of print]

The Fatty Acid Amide Hydrolase in Lymphocytes from Sedentary and Active Subjects.


1 Department of Experimental Medicine & Surgery, Tor Vergata University of Rome, Rome, Italy; 2 Department of Movement, Human and Health Sciences, Foro Italico University of Rome, Rome, Italy; 3 Department of Biomedical Sciences, University of Teramo, Teramo, Italy; 4 Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy; 5 European Center for Brain Research/Santa Lucia Foundation, Rome, Italy.



Endocannabinoids (eCBs) and interleukin (IL)-6 levels change during physical activity, thus suggesting their involvement in the modulation of exercise-related processes like inflammation and energy homeostasis. To investigate whether lifestyle might affect the activity of the eCBs-degrading enzyme fatty acid amide hydrolase (FAAH), active and sedentary subjects were enrolled.


Plasma IL-6 levels and lymphocyte FAAH activity of eight physically active male subjects (39.3 ± 2.9 yrs; BMI = 21.1 ± 0.4), usually practicing aerobic exercise (8.1 ± 1.2 hours per week), and eight sedentary subjects (38.8 ± 3.7 yrs, BMI = 23.1 ± 0.8) were measured. Also in vitro effect of IL-6 was tested on FAAH expression and activity, and on FAAH promoter activity in lymphocytes from sedentary subjects.


Under resting conditions (at least 12 hours from the last exercise), the active group showed plasma IL-6 levels (2.74 ± 0.73 pg/mL) and lymphocyte FAAH activity (215.7 ± 38.5 pmol/min per mg protein) significantly higher than those measured in the sedentary group (0.20 ± 0.02 pg/mL, and 42.0 ± 4.2 pmol/min per mg protein). Increased IL-6 levels paralleled increased FAAH activity, and consistently in vitro treatment of lymphocytes from sedentary individuals with 10 ng/mL IL-6 for 48 hours significantly increased FAAH expression and activity. Transient transfection experiments showed that IL-6 induced the expression of a reporter gene under the control of a CRE (cAMP response element)-like region in the human FAAH promoter. A mutation in the same element abolished IL-6 upregulation, demonstrating that this cytokine regulates FAAH activity at the transcriptional level.


IL-6 leads to activation of the FAAH promoter, thus enhancing FAAH activity that modulates the eCB tone in physically active people.





[PubMed – as supplied by publisher]
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