Skip to main content
Canna~Fangled Abstracts

UV induced changes in proteome of rats plasma are reversed by dermally applied cannabidiol

By October 19, 2021October 23rd, 2021No Comments
. 2021 Oct 19;11(1):20666.

doi: 10.1038/s41598-021-00134-8.

Agnieszka Gęgotek 1, Sinemyiz Atalay 1, Elżbieta Skrzydlewska 2
Affiliations 

Abstract

UV radiation is known to induce a multiple changes in the metabolism of skin-building cells, what can affect the functioning not only neighboring cells, but also, following signal transduction releasing into the blood vessels, the entire body. Therefore, the aim of this study was to analyze the proteomic disturbances occurred in plasma of chronically UVA/UVB irradiated rats and define the effect on these changes of skin topically applied cannabidiol (CBD). Obtained results showed significant changes in the expression of numerous anti-inflammatory and signaling proteins including: NFκB inhibitor, 14-3-3 protein, protein kinase C, keratin, and protein S100 after UV irradiation and CBD treatment. Moreover, the effects of UVA and UVB were manifested by increased level of lipid peroxidation products-protein adducts formation. CBD partially prevented all of these changes, but in a various degree depending on the UV radiation type. Moreover, topical treatment with CBD resulted in the penetration of CBD into the blood and, as a consequence, in direct modifications to the plasma protein structure by creating CBD adducts with molecules, such as proline-rich protein 30, transcription factor 19, or N-acetylglucosamine-6-sulfatase, what significantly changed the activity of these proteins. In conclusion, it may be suggested that CBD applied topically may be an effective compound against systemic UV-induced oxidative stress, but its effectiveness requires careful analysis of CBD’s effects on other tissues of the living organism.

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

References

    1. Gonzaga ER. Role of UV light in photodamage, skin aging, and skin cancer: Importance of photoprotection. Am. J. Clin. Dermatol. 2009;10:19–24. doi: 10.2165/0128071-200910001-00004. – DOI – PubMed
    1. Wondrak GT, Roberts MJ, Jacobson MK, Jacobson EL. 3-Hydroxypyridine chromophores are endogenous sensitizers of photooxidative stress in human skin cells. J. Biol. Chem. 2004;279:30009–30020. doi: 10.1074/jbc.M404379200. – DOI – PubMed
    1. Slominski AT, Zmijewski MA, Plonka PM, et al. How UV light touches the brain and endocrine system through skin, and why. Endocrinology. 2018;159:1992–2007. doi: 10.1210/EN.2017-03230. – DOI – PMC – PubMed
    1. Slominski A, Wortsman J, Luger T, et al. Corticotropin releasing hormone and proopiomelanocortin involvement in the cutaneous response to stress. Physiol. Rev. 2000;80:979–1020. doi: 10.1152/physrev.2000.80.3.979. – DOI – PubMed
    1. Slominski AT, Zmijewski MA, Skobowiat C, et al. Sensing the environment: Regulation of local and global homeostasis by the skin neuroendocrine system. Adv. Anat. Embryol. Cell Biol. 2012;212:v-115. doi: 10.1007/978-3-642-19683-6_1. – DOI – PMC – PubMed

LinkOut – more resources

Leave a Reply